质粒类型：酵母表面展示系统

高拷贝/低拷贝：高拷贝

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：5009 bp 

5' 测序引物及序列：pYD1-F:AGTAACGTTTGTCAGTAATTGC

3' 测序引物及序列：pYD1-R:GTCGATTTTGTTACATCTACAC

载体标签：C-His

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：TRP1

备注：配套酿酒酵母菌株是EBY100.

pYD1为酒精酵母菌表面展示载体，可以通过锚定蛋白将目的蛋白大量展示到酵母细胞壁中，操作简便，产量高。

The pYD1 Yeast Display Vector is specifically designed to target and display recombinant proteins on the surface of Saccharomyces cerevisiae. Displayed proteins can be analyzed for their ability to interact with known or putative ligands. pYD1 uses the a-agglutinin yeast adhesion receptor which consists of two domains, Aga1 and Aga2, to display recombinant protein on the surface of S. cerevisiae. The gene of interest is cloned into the pYD1 vector in frame with the AGA2 gene. The resulting construct is transformed into the EBY100 S. cerevisiae strain (supplied transformed with AGA1). Aga1 and the Aga2-fusion protein associate within the secretory pathway and are displayed on the cell surface.

pYD1 载体说明(点击下载)

pYD1 载体图谱(点击下载)

pYD1质粒序列.txt