产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VJC0487 | pGBKT7-Lam | Clontech |
酵母双杂交系统
|
询价 | 现货 |
质粒类型:酵母双杂交系统
克隆方法:多克隆位点,限制性内切酶
载体大小:7.9kb
5' 测序引物及序列:T7 Forward
载体标签:c-Myc
载体抗性:Kanamycin (卡纳霉素)
载体特点:Matchmaker Two-Hybrid System 3的负对照。
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.