| 产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
|---|---|---|---|---|---|
| HG-VJI0299 | pPIC9K | Invitrogen |
酵母表达载体
|
询价 | 现货 |
载体用途：酵母表达载体；毕赤酵母表达载体
启动子：AOX1 promoter
复制子：pBR322 ori
质粒大小：9276 bp
原核抗性：Amp+、Kan+
筛选标记：HIS4
载体标签：N端α-Factor
克隆菌株：DH5a、TOP10、JM109等常用大肠杆菌
培养条件：37℃
拷贝数（Copy number）：高
表达宿主：酵母细胞
5'测序引物：5'AOX（5'-GACTGGTTCCAATTGACAAGC-3'）
3'测序引物：3'AOX（5'-GGCAAATGGCATTCTGACAT-3'）
备注：利用alpha分泌因子（α-Factor secretion signal），分泌表达目的蛋白。
The pPIC9K vector carries the kanamycin resistance gene that confers resistance to Geneticin? Reagent in Pichia. Spontaneous generation of multiple insertion events can be identified by resistance to increased levels of Geneticin? Reagent. Pichia transformants are selected on histidine-deficient medium and screened for their level of resistance to Geneticin? Reagent. The ability to grow in high concentrations of Geneticin? indicates that multiple copies of the kanamycin resistance gene and the gene of interest are integrated into the genome. The pPIC9K vector directs secretion of expressed proteins.
