产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VJI0556 | pYES2/NT C | Invitrogen |
酵母表达载体
|
询价 | 现货 |
质粒类型:酿酒酵母表达载体
表达水平:高拷贝
诱导方法:半乳糖
启动子:GAL1
克隆方法:多克隆位点,限制性内切酶
载体大小:6037 bp
5' 测序引物及序列:T7:TAATACGACTCACTATAGGG
3' 测序引物及序列:CYC1 Terminator:GTGACATAACTAATTACATGATG
载体标签:N-His, N-Xpress, C-His, C-V5
载体抗性:Ampicillin (氨苄青霉素)
筛选标记:URA3
备注:利用半乳糖诱导蛋白在酿酒酵母中表达。
pYES2/NT and pYES2/CT are S. cerevisiae expression vectors derived from the parental pYES2 vector. Both vectors offer the URA3 gene for selection in yeast. pYES2/NT features an N-terminal Xpress? epitope for detection with Invitrogen's Anti-Xpress? Antibody and a polyhistidine (6xHis) tag for purification with nickel-chelating resin. pYES2/CT contains a C-terminal V5 epitope for detection and a polyhistidine (6xHis) tag for purification.