别名：pEF4 V5-His A, pEF4/V5-HisA, pEF4 V5-HisA

质粒类型：哺乳动物表达载体

高拷贝/低拷贝：高拷贝

启动子：EF-1a

克隆方法：多克隆位点，限制性内切酶

载体大小：5769bp

5' 测序引物及序列：T7 Fwd:5'd[TAATACGACTCACTATAGGG]3'

载体标签：6X His, V5

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Zeocin

备注：该载体易于克隆到其他载体中去

pEF4/V5-His A, B, and C are 5.7 kb vectors derived from pEF1/V5-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain thefollowing elements:

1. Human elongation factor 1a-subunit promoter (hEF-1α) promoter for high-level expression across a broad range of species and cell types (Goldman et al., 1996)(Mizushima and Nagata, 1990).

2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the V5 epitope and a polyhistidine metal-binding peptide.

3. Zeocin resistance gene for selection of stable cell lines (Mulsant et al., 1988).

4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen(e.g. COS7)

The control plasmid, pEF4/V5-His/lacZ is included for use as a positive control for transfection, expression, purification, and detection in the cell line of choice.

pEF4/V5-His A&B&C 载体说明(点击下载)

pEF4/V5-His A&B&C 载体图谱(点击下载)

pEF4V5-His A质粒序列.txt