产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VPI0033 | pVitro2-neo-mcs | Invivogen |
哺乳动物表达载体
|
询价 | 现货 |
质粒类型:哺乳动物表达载体
高拷贝/低拷贝:高拷贝
启动子:CMV
克隆方法:多克隆位点,限制性内切酶
载体大小:6125 bp
5' 测序引物及序列:EF-1a-F:TCAAGCCTCAGACAGTGGTTC
载体抗性:Kanamycin (卡那霉素)
筛选标记:Neo
pVITRO is a family of plasmids developed mainly for in vitro studies. They allow the ubiquitous and constitutive co-expression of two genes of interest.
pVITRO plasmids can be stably transfected in mammalian cells and the genes of interest are expressed at high levels.
Each pVITRO plasmid is available with either two multiple cloning sites or two reporter genes.
More efficient and convenient than using two separate vectors
High levels of expression from two separate transcription units
A choice of selectable markers that are active both in E. coli and mammalian cells
Convenient cloning of cDNAs with two multiple cloning sites (MCS)
Or also available with GFP and LacZ Reporter Genes
Co-expression of two or more genes from a single vector is more efficient and convenient than using two separate vectors. pVITRO are multigenic plasmids that contain two distinct transcription units (TU).
Each pVITRO plasmid features two constitutive promoters that drive high levels of expression from two separate TU in a large number of mammalian cell lines.
Transcriptional interference is minimized by using promoters of different origins or promoters that are coordinately activated (i.e. ferritin promoters), and strong polyadenylation signals (polyA).
pVITRO1 plasmids carry two elongation factor 1 alpha (EF1α) promoters, from rat and mouse origins combined to the CMV and SV40 enhancers respectively.
pVITRO2 plasmids contain two human ferritin composite promoters, FerH (heavy chain) and FerL (light chain), combined to the SV40 and CMV enhancers respectively. To eliminate the iron regulation, their 5‘UTRs have been replaced by the 5‘UTR of the mouse and chimpanzee EF1α genes.
pVITRO plasmids are available with different selectable markers that are active both in E. coli and mammalian cells: bsr (blasticidin resistance), hph (hygromycin resistance), neo (kanamycin/G418 resistance). In bacteria, the resistance gene is expressed from the E. coli EM7 promoter. In mammalian cells, it is transcribed from the promoter located 3‘ of the Ori, as a polycistronic mRNA and translated through the IRES of the Foot and Mouth Disease Virus.
pVITRO-mcs plasmids contain two multiple cloning sites (MCS) for the convenient cloning of cDNAs.
pVITRO-GFP/LacZ express the GFP and LacZ reporter genes, and can be used as controls. As the reporter genes are flanked by unique restriction sites, they can be used for the cloning of open reading frames that can be chosen from InvivoGen‘s Open Reading Frame lists.
pVITRO2 plasmids contain two human ferritin composite promoters, FerH (heavy chain) and FerL (light chain), combined to the SV40 and CMV enhancers respectively. To eliminate the iron regulation, their 5’UTRs have been replaced by the 5’UTR of the mouse and chimpanzee EF-1α genes.
pVITRO2-mcs is available with 2 multiple cloning sites (MCS).
MCS 1 5‘- Age I, Eco RV, Bam HI, Mlu I, Cla I, Sal I, Avr II -3‘
MCS 2 5‘- Sgr AI, Fsp I, Bgl II, Eco RI, Bst BI, Xho I, Nhe I-3‘