| 产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
|---|---|---|---|---|---|
| HG-VRC0347 | RNAi-Ready pSIREN-RetroQ-ZsGreen | Clontech |
RNAi载体
|
询价 | 现货 |
质粒类型：RNAi载体
启动子：Human U6
载体大小：6568 bp
5' 测序引物及序列：U6:5'-ATGGACTATCATATGCTTACCGTA-3'
载体抗性：Ampicillin (氨苄青霉素)
RNAi-Ready pSIREN-RetroQ-ZsGreen derives from our original gene silencing retroviral vector RNAi-Ready pSIREN-RetroQ. pSIREN-RetroQ-ZsGreen is a self-inactivating retroviral expression vector designed to express a small hairpin RNA (shRNA) using the human U6 promoter. This vector is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when an annealed DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. The pSIREN-RetroQ-ZsGreen construct can be transfected as a plasmid expression vector. For generation of active retrovirus for infecting hard-to-transfect cell lines, transfect the pSIREN-RetroQ-ZsGreen construct into a retroviral packaging cell line and collect the viral supernatant.
