| 产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
|---|---|---|---|---|---|
| HG-VYN0197 | pCDFDuet-1 | Novagen |
原核表达载体
|
询价 | 现货 |
载体名称：pCDFDuet-1，pCDFDuet1，pCDFDuet 1
质粒类型：大肠杆菌表达载体（双启动子）
表达水平：高
克隆方法：多克隆位点，限制性内切酶
载体大小：3781 bp
5' 测序引物：T7（5'-TAATACGACTCACTATAGGG-3'）
3' 测序引物：T7-ter（5'-TGCTAGTTATTGCTCAGCGG-3'）
载体标签：N-His, C-S
载体抗性：Streptomycin
备注：pCDFDuet-1 is designed for the coexpression of two target genes. pCDFDuet-1 carries the CloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance gene (aadA).
pCDFDuet-1 is designed for the coexpression of two target ORFs. It encodes two MCS each of which is preceded by a T7 promoter, lac operator, and rbs. The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. It can be used in combination with pACYCDuet-1, pRSFDuet-1, and pETDuet-1 in an appropriate host strain for the coexpression of 4 to 8 target proteins. Plasmids that share common origins of replication or antibiotic resistance genes should not be used for the coexpression of two proteins.
