产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VDW0535 | XL2-Blue MRF’ |
克隆菌株
|
询价 | 现货 |
出品公司:Stratagene
菌株类型:E.coli
培养基:LB培养基
生长条件:37℃,有氧
基因型:D(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F¢proAB lacIqZDM15 Tn10 (Tetr) Amy Camr]
抗性:氯霉素,四环素
质粒转化条件:42℃热激
应用:蛋白表达
XL2-Blue MRF´ ultracompetent cells are high-efficiency derivatives of our XL1-Blue MRF´ supercompetent cells. Using ultracompetent cell technology, we have achieved transformation efficiencies of greater than 5 × 109 transformants/µg of pUC18 DNA, making these ultracompetent cells an ideal choice for high-efficiency cloning. The XL2-Blue MRF´ (Minus Restriction) strain is deficient in all known restriction systems (McrA–, McrCB–, McrF–, Mrr–, HsdR–), preventing cleavage of cloned DNA that carries cytosine and/or adenine methylation, which is often present in eukaryotic DNA and cDNA. XL2-Blue MRF´ cells are restriction minus. The strain is endonuclease deficient (endA), greatly improving the quality of miniprep DNA, and recombination deficient (recA), helping to ensure insert stability. These cells allow blue-white screening for recombinant plasmids.