| 产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
|---|---|---|---|---|---|
| HG-VJA0316 | YEp352（pYEp352） | ATCC |
酵母表达载体
|
询价 | 现货 |
载体名称：YEp352；pYEp352
载体用途：酵母表达载体；酿酒酵母表达载体
质粒大小：5181bp
启动子：URA3 promoter
复制子：2μ ori
原核抗性：Amp+
筛选标记：URA3
克隆菌株：DH5a
培养条件：37℃
表达宿主：酵母细胞
测序引物：M13-f、M13-r
Constructed by filling in and blunt-end ligating pUC18 linearized at NdeI, a 1.4 kb RsaI fragment of the 2 micron circle, and a 1.1 kb HindIII/SmaI fragment containing URA3. A XbaI site in 2u and a PstI site in URA3 were removed. The yeast genomic sequence is in nt 286-1383 (where the A of the ATG of lacZ' is number 1), the 2 micron sequence goes from nt 1384-2768, & pUC18 includes nt 2769-285. Restriction digests of the clone give the following sizes (kb): BamHI--5.3; XbaI--5.3; PstI--5.3; HindIII--5.3. (ATCC staff) This is a E.coli/yeast episomal shuttle vector with multiple restriction sites for cloning. (personal communication) Medium is 1227 LB plus ampicillin. NCBI gi: 295271 Hosts: E.coli RR1, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pBR322, 2micron.
