质粒类型：酵母双杂交系统

启动子：ADH1

克隆方法：多克隆位点，限制性内切酶

载体大小：8117 bp 

载体抗性：Ampicillin (氨苄青霉素)

pACT2 generates a fusion of the GAL4 AD (amino acids 768881), a HA epitope tag, and a protein of interest (or protein encoded by a cDNA in a fusion library) cloned into the MCS in the correct orientation and reading frame. pACT2, which is derived from pACT, contains a unique EcoR I site in the MCS. The hybrid protein is expressed at high levels in yeast host cells from the constitutive ADH1 promoter (P); transcription is terminated at the ADH1 transcription termination signal (T). The protein is targeted to the yeast nucleus by the nuclear localization sequence from SV40 T-antigen which has been cloned into the 5' end of the GAL4 AD sequence. pACT2 is a shuttle vector that replicates autonomously in both E. coli and S. cerevisiae and carries the bla gene, which confers ampicillin resistance in E. coli. pACT2 also contains the LEU2 nutritional gene that allows yeast auxotrophs to grow on limiting synthetic media. Transformants with AD/library plasmids can be selected by complementation by the LEU2 gene by using an E. coli strain that carries a leuB mutation (e.g., HB101).

pACT2 AD 载体说明(点击下载)

pACT2 AD质粒序列.txt

pACT2 AD 载体序列(点击下载)