质粒类型：酵母三杂交系统

启动子：ADH1

克隆方法：多克隆位点，限制性内切酶

载体大小：6526 bp 

5' 测序引物及序列：Match:5'd[TCATCGGAAGAGAGTAGT]3'

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：TRP1

备注：Used as bait (& bridge) for yeast 3-hybrid screen.

pBridge expresses two proteins: a DNA-binding domain fusion, and an additional protein. pBridge thus allows establishment of three-hybrid systems when used in combination with an activation domain fusion vector and yeast strains from any of Clontech's GAL4-based two-hybrid systems, including Matchmaker? Gold. This vector generates a hybrid protein that contains the sequences for the GAL4 DNA-binding domain (DNA-BD) and the sequence cloned into MCS I. The fusion protein is expressed in yeast host cells from the constitutive ADH1 promoter; transcription is terminated at the ADH1 transcription termination signal. The hybrid protein is targeted to the yeast nucleus by nuclear localization sequences (NLS) that are an intrinsic part of the GAL4 DNA-BD. An additional gene of interest can be cloned into MCS II which is located downstream of an HA epitope and a second NLS. The resulting fusion protein is conditionally expressed from the Met25 promoter in response to methionine levels in the medium; i.e., it is repressed in the presence of 1 mM methionine and expressed in the absence of methionine.

pBridge 载体说明(点击下载)

pBridge质粒序列.txt

pBridge 载体序列(点击下载)