质粒类型：酿酒酵母表达载体

表达水平：高拷贝

诱导方法：半乳糖

启动子：GAL1 promoter

克隆方法：多克隆位点，限制性内切酶

载体大小：5870 bp

5' 测序引物及序列：GAL1-F: AATATACCTCTATACTTTAACGTC

3' 测序引物及序列：CYC1-R: GCGTGAATGTAAGCGTGAC

载体标签：C-His, C-V5

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：TRP1

备注：利用半乳糖诱导蛋白在酿酒酵母中表达

pYES3/CT and pYES6/CT are S. cerevisiae expression vectors derived from the parental pYES2 vector. Vectors feature a C-terminal V5 epitope for detection with an Anti-V5 Antibody. pYES3/CT carries the TRP1 selection marker for selection in yeast. pYES6/CT carries the bsd resistance gene for selection with the antibiotic Blasticidin, a potent selection agent that can be used at very low concentrations and in any strain regardless of auxotrophic marker.

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