质粒类型：慢病毒载体

高拷贝/低拷贝：高拷贝

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：7881 bp 

5' 测序引物及序列：LKO.1 5’:GACTATCATATGCTTACCGT

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：ZsGreen1

备注：含有人U6启动子的慢病毒shRNA载体

pLVX-shRNA2 is an HIV-1-based, lentiviral expression vector designed to express a small hair-pin RNA (shRNA) for RNA interference (RNAi) studies. Expression of your shRNA is driven by the RNA Pol III-dependent, human U6 promoter (PU6), located just upstream of the MCS. pLVX-shRNA2 can be used as a plasmid expression vector and transfected into cells, or it can be packaged into viral particles and transduced into cells. Lentiviral particles derived from the vector allow the expression of shRNAs in virtually any cell type, including primary cells.

pLVX-shRNA2 载体说明(点击下载)

pLVX-shRNA2质粒序列.txt