质粒类型：逆转录病毒载体

高拷贝/低拷贝：低拷贝

启动子：LTR

克隆方法：多克隆位点，限制性内切酶

载体大小：6264 bp 

5' 测序引物及序列：MSCV-F:CCCTTGAACCTCCTCGTTCGACC

3' 测序引物及序列：pMSCV-R:GAGACGTGCTACTTCCATTTGTC

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Hygromycin（潮霉素）

The Murine Stem Cell Virus (MSCV) vectors were derived from the Murine Embryonic Stem Cell Virus (MESV) and the LN retroviral vectors (1,2). Upon transfection into a packaging cell line, pMSCVpuro transiently expresses, or integrates and stably expresses, a transcript containing the extended viral packaging signal Ψ+, the puromycin resistance gene, and a gene of interest. The vectors achieve stable, high-level gene expression in hematopoietic and embryonic stem cells through a specifically designed 5' long terminal repeat (LTR). This LTR is from the murine stem cell PCMV virus, and it differs from the MoMuLV LTR used in other retroviral vectors by several point mutations and a deletion. These changes enhance transcriptional activation and prevent transcriptional suppression in embryonic stem and embryonal carcinoma cells. As a result, the LTR drives high-level constitutive expression of a target gene in stem cells or other mammalian cell lines (3). A gene can be cloned into the multiple cloning site immediately downstream of this LTR. The murine phosphoglycerate kinase (PKG) promoter (PPKG) controls expression of the puromycin resistance gene (Puror) for antibiotic selection in eukaryotic cells. pMSCVpuro also contains the pUC origin of replication and E. coli Ampr gene for propagation and antibiotic selection in bacteria.

pMSCVpuro 载体说明(点击下载)

pMSCVpuro质粒序列.txt

pMSCVpuro 载体序列(点击下载)