产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VPI0009 | pcDNA3.1/Zeo(+) | Invitrogen |
哺乳动物表达载体
|
询价 | 现货 |
质粒类型:哺乳动物表达载体
启动子:CMV
表达水平:高
克隆方法:多克隆位点,限制性内切酶
载体大小:5015 bp (查看载体序列)
5' 测序引物: pcDNA3.1-F:CTAGAGAACCCACTGCTTAC
3' 测序引物: pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG
载体抗性:Ampicillin (氨苄青霉素)
筛选标记:Zeocin 博来霉素
备注:
Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.
This pcDNA™3.1/Zeo(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin™ selectable marker and a forward-orientation multiple cloning site.
Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
Cytomegalovirus (CMV) enhancer-promoter for high-level expression
Large multiple cloning site in either forward (+) or reverse (-) orientations
Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e.,COS-1 and COS-7)
Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
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