质粒类型：哺乳动物表达载体

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：5502bp

5' 测序引物及序列：T7 Fwd:5'd[TAATACGACTCACTATAGGG]3'

载体标签：His Tag (6x), V5 Epitope Tag

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Neo (新霉素)

        pcDNA ™ 3.1/V5-His A, B, and C are 5.5 kb vectors derived from pcDNA ™ 3.1(+) and designed for high-level expression, purification, and detection of recombinant proteins in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells.

        The vectors contain the following elements:

（1）Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the V5 epitope and a polyhistidine metal-binding peptide

（2）Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells

（3）Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7) 

        The control plasmid, pcDNA ™ 3.1/V5-His/lacZ, contains a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. pcDNA ™ 3.1/V5-His/lacZ is included for use as a positive control for transfection, expression, purification, and detection in the cell line of choice.

pcDNA3.1/V5-His A&B&C载体使用(点击下载)

pcDNA3.1V5-His C质粒序列.txt