产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VPI0018 | pcDNA3.1(-)/myc-His B | Invitrogen |
哺乳动物表达载体
|
询价 | 现货 |
别名:pcDNA3.1/myc-His(-) B
质粒类型:哺乳动物表达载体
表达水平:高拷贝
启动子:CMV
克隆方法:多克隆位点,限制性内切酶
载体大小:5497bp
5' 测序引物及序列:T7 Fwd:5'd[TAATACGACTCACTATAGGG]3'
载体标签:6X His, myc
载体抗性:Ampicillin (氨苄青霉素)
筛选标记:新霉素(Neo)
最常用的哺乳动物表达载体之一。
CMV promoter强启动子调控外源基因表达,C端携带myc标签及His标签,方便进行表达检测。
载体拷贝数很高,表达量也很高。
无荧光标记。
Amp+原核筛选抗性,Neo+真核筛选抗性,可以利用G418筛选稳定细胞株。
pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.