质粒类型:RNAi载体

启动子:CMV

克隆方法:多克隆位点，限制性内切酶

载体大小:4944bp 

5' 测序引物及序列:5'-AGGCGATTAAGTTGGGTA-3'

3' 测序引物及序列:5'-CGGTAGGCGTGTACGGTG-3'

载体抗性:Ampicillin (氨苄青霉素)

筛选标记:Neomycin (新霉素)

The pSilencer 4.1-CMV siRNA Expression vectors are linearized with both BamH 1 and Hind III to facilitate directional cloning. They are also purified to remove the digested insert so that it cannot re-ligate with the vector. This greatly increases the percentage of clones bearing the hairpin siRNA template insert after ligation, reducing the time and effort required to screen clones.

pSilencer 4.1-CMV neo 载体说明(点击下载)

pSilencer 4.1-CMV neo质粒序列.txt