产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VRA0343 | pSilencer 4.1-CMV puro | Ambion |
RNAi载体
|
询价 | 现货 |
质粒类型:RNAi载体
启动子:CMV
克隆方法:多克隆位点,限制性内切酶
载体大小:4781bp
5' 测序引物及序列:5'-AGGCGATTAAGTTGGGTA-3'
3' 测序引物及序列:5'-CGGTAGGCGTGTACGGTG-3'
载体抗性:Ampicillin (氨苄青霉素)
筛选标记:Puromycin
The pSilencer 4.1-CMV siRNA Expression vectors are linearized with both BamH 1 and Hind III to facilitate directional cloning. They are also purified to remove the digested insert so that it cannot re-ligate with the vector. This greatly increases the percentage of clones bearing the hairpin siRNA template insert after ligation, reducing the time and effort required to screen clones.