质粒类型：RNAi载体

高拷贝/低拷贝：低拷贝

启动子：H1

克隆方法：多克隆位点，限制性内切酶

载体大小：6253bp 

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Puromycin（嘌呤霉素）

备注：逆病毒基因沉默载体，能够将发卡的siRNA转染进入大多数哺乳细胞中。

pSilencer 5.1 Retro vectors harness retroviruses’ ability to stably integrate into the host genome of nearly all mitotically active cells and include a puromycin resistance gene for selection of cells that express the recombinant DNA. The pSilencer 5.1 Retro vectors enable researchers to study the long-term effects of reducing the expression of specific genes in cell culture models. This technology expands the capabilities of RNA interference research to address long-term alteration of gene expression.
pSilencer 5.1-H1 Retro uses the H1 promoter.

pSilencer5.1-U6 Retro 载体说明(点击下载)

pSilencer5.1-H1 Retro质粒序列.txt