质粒类型：四环素调控系统

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：7139 bp 

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Neomycin（新霉素）

pTet-On-Advanced expresses an improved version of the reverse Tet (tetracycline)-controlled transactivator protein (rtTA), called rtTA-Advanced (1–4). It is more sensitive to doxycycline (Dox) and yields lower background expression than the original rtTA used in the Tet-On® System (2). The rtTA-Advanced protein is a fusion of amino acids 1–207 of a mutant Tet repressor (TetR) and 39 amino acids containing three minimal "F"-type transcriptional activation domains from the VP16 protein of herpes simplex virus. It is fully synthetic, lacks cryptic splice sites, and is codon-optimized for stable expression in mammalian cells.

pTet-on advanced产品说明书.pdf

pTet-on advanced质粒序列.txt

pTet-on advanced 载体序列(点击下载)