质粒类型：腺病毒载体

启动子：无启动子

克隆方法：Gateway Cloning

载体大小：34864 bp 

载体抗性：Ampicillin，Chloramphenicol

The pAd⁄PL-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ adenoviral expression vector, pAd⁄PL-DEST® vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where expression is driven by a promoter of choice. Alternatively, the vector may also be used to express small RNA molecules from their appropriate promoters.

Advantages
• High efficiency and rapid recombination cloning
• Produces high titer adenoviral stocks
• Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
• Allows gene of interest to be controlled by a promoter of choice
• Produces replication-incompetent virus for enhanced biosafety of the system
• Amenable for use in high-throughput applications

Key Features
• Gateway® Technology for efficient and rapid cloning
• Promoterless vector that allows gene of interest to be controlled by a promoter of choice 
• Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
• Ampicillin selection marker

pAd/PL-DEST 载体说明(点击下载)

pAd/PL-DEST 多聚接头(点击下载)

pAd/PL-DEST 限制性酶切(点击下载)

pAd/PL-DEST 载体图谱(点击下载)

pAdPL-DEST（骨架载体)质粒序列.txt

pAd/PL-DEST 载体序列(点击下载)