载体别名：pET40b, pET 40b

质粒类型：大肠杆菌表达载体

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：6190 bp

5' 测序引物：T7

3' 测序引物：T7-ter

载体标签：His (中间和C端), N-DsbC, N-S, N-EK, N-Thrombin

载体抗性：Kanamycin (卡那霉素)

备注：Encodes DsbC tag for export and periplasmic folding of target proteins.

The pET-40b(+) vector is designed for expression of DsbC fusion proteins. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/ expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer.

pET-40b(+)(点击下载)

pET-40b(+)质粒序列.txt