质粒名称：pET-44a(+)，pET-44a，pET44a，pET 44a

质粒类型：大肠杆菌表达载体

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：7311 bp 

5' 测序引物：T7

5' 测序引物序列：T7:5'-TAATACGACTCACTATAGGG-3'

3' 测序引物：ColiDOWN

3' 测序引物序列：ColiDOWN:5'-TTCACTTCTGAGTTCGGCATG-3'

载体标签：C-HSV, N-His, C-His, N-Thrombin, N-Nus, N-EK

载体抗性：Ampicillin (氨苄青霉素)

备注：

Both Nterm and Cterm His tags; Nterm thrombin cleavage site; Nterm enterokinase cleavage site; a,b,c vary by MCS.

The pET-44 vectors are designed for cloning and high-level expression of peptide sequences fused with the 495 aa Nus•Tag™ protein. Compared to the pET-43.1 series, the pET-44 vectors encode an additional N-terminal His•Tag. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the COLIDOWN primer (Cat. No. 70845-3). Vector encoded sequence can be completely removed when cloning into the PshA I or Sma I sites (as shown below) by cleaving the Nus•Tag fusion protein with enterokinase or thrombin, respectively.

pET-44a(+) 载体说明(点击下载)

pET-44a(+)质粒序列.txt