质粒类型：哺乳动物表达载体

启动子：CMV

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：3114 bp 

5' 测序引物：CMV-F

5' 测序引物序列:5'-CGCAAATGGGCGGTAGGCGTG-3'

载体抗性：Ampicillin (氨苄青霉素)

pBI-CMV1 is a mammalian bidirectional expression vector that allows the constitutive expression of two proteins of interest. Protein expression is driven by one of two constitutively active, minimal human cytomegalovirus promoters, PminCMV1 and PminCMV2, located just upstream of two independent multiple cloning sites (MCS 1 and MCS 2, respectively). To allow propagation and selection in E. coli, the vector also contains a ColE1 origin of replication and an ampicillin resistance gene (Ampr). 

Use: pBI-CMV1 is designed to simultaneously and constitutively express two genes of interest. Each gene must be cloned into a different MCS, and contain both an initiation codon and a stop codon. The pBI-CMV1 vector can be transfected into mammalian cells using any standard transfection method.

pBI-CMV1产品说明书.pdf

pBI-CMV1质粒序列.txt