Y187 is used in the construction of our Mate & Plate and normalized Mate & Plate Libraries, which are recommended for use with the Matchmaker Gold Yeast Two-Hybrid System. These complex, tissue-specific, ready-to-go libraries are constructed directly in our Y187 library yeast stain, utilizing the highly efficient homologous recombination machinery of Saccharomyces cerevisiae. Mate & Plate Libraries provide, by far, the simplest method for yeast two-hybrid screening because they do not require library-scale transformations or labor-intensive library amplification.

You can also use the Y187 Yeast Strain with our convenient Make Your Own "Mate & Plate" Library System to construct your own yeast two-hybrid libraries, and eliminate the time-consuming and labor-intensive library cloning, amplification, and harvesting in E. coli that are required by traditional methods of library construction.

MATCHMAKER GAL4 Two-Hybrid System 3 & Libraries User Manual(点击下载)

Yeast Protocols Handbook(点击下载)