产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VJA1079 | pRS403 | ATCC |
酵母表达载体(整合型)
|
询价 | 现货 |
载体用途:酵母表达载体(整合型);酿酒酵母表达载体
质粒大小:4456bp
原核抗性:Amp+
筛选标记:HIS3
复制子:pUC ori
克隆菌株:DH5a
培养条件:37℃
表达宿主:YPH499、YPH500等酿酒酵母
5'测序引物:T7(5'-TAATACGACTCACTATAGGG-3')
3'测序引物:M13-R(5'-CAGGAAACAGCTATGACC-3')
pRS4xx系列质粒被用于基因的穿梭载体克隆酿酒酵母,但能够在大肠杆菌中复制。虽然在大肠杆菌中,他们从pMB1的复制原点复制至pBR322。他们携带的bla(AmpR)标记用于选择用氨苄青霉素和复制的噬菌体F1原点单链DNA 。
pRS403 through pRS406 are integrative plasmids (YIp); pRS413through pRS416 (shown below) are centromeric plasmids (YCp),and differ from the YIp by the insertion of a centromere (CEN)and autonomously replicating sequence (ARS); pRS423 throughpRS426 are episomal plasmids (YEp), and carry instead theorigin of replication from the yeast 2µ circle plasmid along withREP3 and FRT sequences. The 4 vectors in each series differonly in the yeast auxotrophic marker used: HIS3 (pRS4x3), TRP1(pRS4x4), LEU2 (pRS4x5) or URA3 (pRS4x6).
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