| 产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
|---|---|---|---|---|---|
| HG-VCA1525 | pC013-Twinstrep-SUMO-hulwCas13a (Plasmid #90097) | Addgene |
CRISPR/Cas系统
|
询价 | 现货 |
Vector backbone：pET
Backbone size w/o insert (bp)：6086
Total vector size (bp)：9542
Vector type：Bacterial Expression
Bacterial Resistance(s)：Ampicillin
Growth Temperature：37°C
Growth Strain(s)：Rosetta 2(DE3)pLysS Singles
Copy number：Low Copy
Gene/Insert name：LwCas13a
Alt name：LwC2c2
Alt name：Cas13a
Alt name：C2c2
Species：Synthetic; Leptotrichia wadeii
Insert Size (bp)：3280
Tag / Fusion Protein：6x His Tag/Twin Strep/SUMO (N terminal on insert)
Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.
参考文献：
Nucleic acid detection with CRISPR-Cas13a/C2c2. Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F. Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321. 10.1126/science.aam9321 PubMed 28408723
具体载体资料可参考Addgene官网：
https://www.addgene.org/90097/
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