ID pcDNA3.1(hyg)+ standard; circular DNA; ; 5597 BP. CC This file is created by Vector NTI CC VNTDATE|609865418| CC VNTDBDATE|706983202| CC LSOWNER| CC VNTAUTHORNAME| HonorGene | www.honorgene.com FH Key Location/Qualifiers FH FT promoter 209..863 FT /vntifkey="29" FT /label=CMV\promoter FT polyA_signal 1021..1235 FT /vntifkey="25" FT /label=BGH\polyA FT rep_origin 1298..1711 FT /vntifkey="33" FT /label=f1\ori FT rep_origin 1776..2100 FT /vntifkey="33" FT /label=SV40\ori FT CDS 2118..3143 FT /vntifkey="4" FT /label=hygro+ FT polyA_signal 3154..3526 FT /vntifkey="25" FT /label=SV40\polyA FT rep_origin complement(3786..4456) FT /vntifkey="33" FT /label=pUC\ori FT CDS complement(4601..5461) FT /vntifkey="4" FT /label=Amp+ FT primer 769..789 FT /vntifkey="27" FT /label=CMV\forward\primer FT primer 1022..1039 FT /vntifkey="27" FT /label=BGH\reverse\primer SQ Sequence 5597 BP; 1301 A; 1451 C; 1432 G; 1413 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 960 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca 1020 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 1080 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 1140 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 1200 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 1260 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 1320 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 1380 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 1440 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 1500 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 1560 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 1620 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 1680 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 1740 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 1800 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 1860 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 1920 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 1980 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 2040 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 2100 gatctgatca gcacgtgatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc 2160 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc 2220 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg 2280 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc 2340 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg 2400 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg 2460 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc 2520 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg 2580 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg 2640 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg 2700 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca 2760 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct 2820 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg 2880 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct 2940 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg 3000 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg 3060 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca 3120 ctcgtccgag ggcaaaggaa tagcacgtgc tacgagattt cgattccacc gccgccttct 3180 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 3240 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt 3300 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 3360 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta 3420 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 3480 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 3540 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 3600 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 3660 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 3720 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 3780 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 3840 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 3900 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 3960 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 4020 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 4080 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 4140 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 4200 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 4260 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 4320 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 4380 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 4440 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 4500 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 4560 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 4620 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 4680 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 4740 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 4800 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 4860 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 4920 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 4980 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 5040 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 5100 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 5160 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 5220 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 5280 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 5340 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 5400 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 5460 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 5520 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 5580 aagtgccacc tgacgtc 5597 //