ID pBiFC-VC155 standard; circular DNA; SYN; 4088 BP. AC .; DT 06-AUG-2018 DE Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays. KW pBiFC-VC155. OS synthetic DNA construct CC GBRFLN|REFERENCE 1 (bases 1 to 4088) CC GBRFLN| AUTHORS Shyu YJ, Liu H, Deng X, Hu CD CC GBRFLN| TITLE Identification of new fluorescent protein fragments for bimolecular CC GBRFLN| fluorescence complementation analysis under physiological CC GBRFLN| conditions. CC GBRFLN| JOURNAL Biotechniques. 2006 Jan . 40(1):61-6. CC This file is created by Vector NTI CC http://www.honorgene.com/ CC VNTAUTHORNAME | HonorGene | CC VNTAUTHORTEL | Tel：15574926881 | CC VNTAUTHOREML | Email：12695920@qq.com | FH Key Location/Qualifiers FH FT source 1..4088 FT /organism="synthetic DNA construct" FT /mol_type="other DNA" FT /vntifkey="98" FT enhancer 44..347 FT /vntifkey="9" FT /label=CMV\enhancer FT /note="human cytomegalovirus immediate early enhancer" FT promoter 348..551 FT /vntifkey="30" FT /label=CMV\promoter FT /note="human cytomegalovirus (CMV) immediate early promoter" FT intron 689..785 FT /vntifkey="15" FT /label=SV40\intron FT /note="modified SV40 intron with splice donor and acceptor sites" FT CDS 849..875 FT /codon_start=1 FT /product="HA (human influenza hemagglutinin) epitope tag" FT /vntifkey="4" FT /label=HA FT CDS 978..1229 FT /codon_start=1 FT /product="C-terminal fragment of mVenus for use in FT bimolecular fluorescence complementation (BiFC) (Kodama and FT Hu, 2010)" FT /vntifkey="4" FT /label=VC155 FT polyA_signal 1244..1378 FT /vntifkey="25" FT /label=SV40\poly(A)\signal FT /note="SV40 polyadenylation signal" FT rep_origin complement(1891..2479) FT /direction=LEFT FT /vntifkey="33" FT /label=ori FT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" FT CDS complement(2650..3510) FT /codon_start=1 FT /gene="bla" FT /product="beta-lactamase" FT /vntifkey="4" FT /label=AmpR FT /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" FT promoter complement(3511..3615) FT /gene="bla" FT /vntifkey="30" FT /label=AmpR\promoter SQ Sequence 4088 BP; 1031 A; 1035 C; 1028 G; 994 t; gtaaaacgac ggccagtgag ttcgagcttg catgcctgca ggtcgttaca taacttacgg 60 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 120 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 180 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 240 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 300 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 360 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 420 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 480 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 540 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 600 acctccatag aagacaccgg gaccgatcca gcctccggac tctagaggat ccggtactag 660 aggaactgaa aaaccagaaa gttaactggt aagtttagtc tttttgtctt ttatttcagg 720 tcccggatcc ggtggtggtg caaatcaaag aactgctcct cagtggatgt tgcctttact 780 tctaggcctg tacggaagtg ttacttctgc tctaaaagct gcggaattgt acccgcgggc 840 ccaccatgta cccatacgat gttccagatt acgctcttat ggccatggag gcccgaattc 900 ggtcgaccga gatctctcga ggtacccgtc cggcgtgcaa aatcccgaac gacctgaaac 960 agaaagtcat gaaccacgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc 1020 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 1080 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc aaactgagca 1140 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 1200 tcactctcgg catggacgag ctgtacaagt aagcggccgc ggggatccag acatgataag 1260 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg 1320 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa 1380 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggttttttc 1440 ggatcctcta gagtcgatct gcaggcatgc tagcttggcg taatcatggt catagctgtt 1500 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 1560 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 1620 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 1680 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 1740 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 1800 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 1860 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 1920 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 1980 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 2040 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 2100 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 2160 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 2220 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 2280 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 2340 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 2400 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 2460 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 2520 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 2580 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 2640 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 2700 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 2760 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 2820 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 2880 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 2940 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 3000 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 3060 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 3120 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 3180 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 3240 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 3300 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 3360 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 3420 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 3480 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 3540 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 3600 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 3660 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 3720 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 3780 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 3840 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 3900 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 3960 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 4020 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 4080 acgacgtt 4088 //