ID pLVX-IRES-Puro standard; circular DNA; ; 8140 BP. DT 8-NOV-2009 CC Created by HonorGene：http://www.honorgene.com/ CC Cat. No. 632183,632186 CC This file is created by Vector NTI CC ORIGDB|GenBank CC VNTDBDATE|631471593| CC LSOWNER| CC VNTAUTHORNAME| HonorGene | http://www.honorgene.com FH Key Location/Qualifiers FH FT LTR 1..635 FT /vntifkey="19" FT /label=5'\LTR FT misc_feature 685..822 FT /vntifkey="21" FT /label=packaging\signal FT misc_feature 2028..2151 FT /vntifkey="21" FT /label=cPPT/CTS\(DNA\flap) FT promoter 2185..2787 FT /vntifkey="30" FT /label=CMV\IE\Promoter FT misc_feature 4062..4653 FT /vntifkey="21" FT /label=WPRE FT rep_origin complement(5961..6634) FT /vntifkey="33" FT /label=pUC\origin FT CDS complement(6779..7775) FT /vntifkey="4" FT /label=Amp(R) FT misc_binding 1303..1536 FT /vntifkey="20" FT /label=RRE FT misc_binding 636..653 FT /vntifkey="20" FT /label=PBS FT LTR 4856..5492 FT /vntifkey="19" FT /label=3'\LTR FT misc_feature 2841..3448 FT /vntifkey="21" FT /label=IRES FT CDS 3449..4048 FT /vntifkey="4" FT /label=Puro(R) FT misc_feature 2803..2840 FT /vntifkey="21" FT /label=MCS SQ Sequence 8140 BP; 2082 A; 2022 C; 2094 G; 1942 t; tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 60 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 120 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 180 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 240 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 300 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 360 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 420 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 480 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 540 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 600 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 660 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 720 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 780 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 840 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 900 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 960 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1020 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1080 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1140 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1200 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1260 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1320 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1380 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1440 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1500 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1560 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1620 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1680 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1740 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1800 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1860 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1920 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1980 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2040 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2100 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2160 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2220 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2280 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2340 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2400 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2460 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2520 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2580 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2640 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2700 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2760 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt 2820 tctagagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt actggccgaa 2880 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 2940 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 3000 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 3060 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 3120 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 3180 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 3240 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 3300 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 3360 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt 3420 gccacaaccc acaaggagac gaccttccat gaccgagtac aagcccacgg tgcgcctcgc 3480 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 3540 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga 3600 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 3660 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 3720 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 3780 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 3840 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 3900 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 3960 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 4020 gtgcatgacc cgcaagcccg gtgcctagac gcgtctggaa caatcaacct ctggattaca 4080 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 4140 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 4200 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 4260 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 4320 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 4380 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 4440 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 4500 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 4560 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 4620 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga 4680 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct 4740 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc 4800 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga 4860 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca 4920 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac 4980 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa 5040 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag 5100 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct 5160 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg 5220 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg 5280 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct 5340 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 5400 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 5460 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 5520 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt 5580 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 5640 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 5700 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 5760 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 5820 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 5880 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 5940 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 6000 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 6060 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 6120 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 6180 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 6240 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 6300 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 6360 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 6420 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 6480 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 6540 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 6600 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 6660 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 6720 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 6780 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 6840 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 6900 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 6960 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 7020 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 7080 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 7140 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 7200 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 7260 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 7320 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 7380 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 7440 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 7500 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 7560 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 7620 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 7680 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 7740 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt 7800 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 7860 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 7920 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct 7980 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta 8040 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa 8100 gaaattgtat ttgttaaata tgtactacaa acttagtagt 8140 //