LOCUS pVE5523 8605 bp ds-DNA circular SYN 27-8月-2021 DEFINITION . ACCESSION . VERSION . KEYWORDS pVE5523 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8605) AUTHOR Name | HonorGene: http://www.honorgene.com AUTHOR Tel | 15574926881 | AUTHOR Email | 12695920@qq.com | FEATURES Location/Qualifiers source 1..8605 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 186..774 /direction=RIGHT /note="ori" /note="color: #ffff00" protein_bind 1062..1083 /note="CAP binding site" /note="color: #31849b" promoter 1098..1128 /note="lac promoter" /note="This forward directional feature has 3 segments: ???1:?1098?..?1103?/?#ffffff?/?-35 ???2:?1104?..?1121?/?#ffffff ???3:?1122?..?1128?/?#ffffff?/?-10" protein_bind 1136..1152 /note="lac operator" /note="color: #31849b" primer_bind 1160..1176 /note="M13 rev" /note="color: #a020f0; direction: RIGHT" promoter 1197..1215 /note="T3 promoter" /note="color: #ffffff; direction: RIGHT" promoter 1291..1401 /note="P59" /note="Streptococcus cremoris promoter 59 GenBank: M24806" /note="color: #ffffff" misc_feature 1479..1592 /note="PSusp" /note="color: #ccffcc; direction: RIGHT" misc_feature 1599..2056 /note="nucA" /note="color: #ffff99; direction: RIGHT" terminator 2084..2170 /note="rrnB T1 terminator" /note="color: #ffffff" terminator 2189..2216 /note="rrnB T2 terminator" /note="color: #ffffff" CDS 2833..3570 /codon_start=1 /note="Ery+" /note="color: #ccffcc" /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN LSTVTYEQVLSIFNSYLLFNGRK" CDS 4888..6378 /codon_start=1 /note="rep" /note="color: #993366" /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRSK SSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQINTF FIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSEFKS VKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQDWSFK QTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTLSLAYF SSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILCKAWVSS DLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVTTKKEIRE VLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKKEEKESYIK ALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG" promoter 7365..7469 /note="AmpR promoter" /note="color: #ffffff; direction: RIGHT" CDS 7470..8330 /codon_start=1 /note="Amp+" /note="This feature has 2 segments: ???1:?7470?..?7538?/?#ccffcc?/?signal?sequence ???2:?7539?..?8330?/?#ccffcc Cleavage site after base 7538" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ORIGIN 1 tgattagcat tggtactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 61 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 121 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 181 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 241 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 301 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 361 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 421 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 481 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 541 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 601 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 661 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 721 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 781 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 841 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 901 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 961 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 1021 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 1081 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 1141 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcgaaatt 1201 aaccctcact aaagggaaca aaagctggta ccgggccccc cctcgacggt atcgatagcc 1261 cgcctaatga gcgggctttt ttttgatccc ctaaccaaag aagcgcgtaa tatcagggtt 1321 taagagacaa taagaaaaaa agattgaaaa agtgacatta aattcttgac agggagagat 1381 aggtttgata gaatataata ggggtaccga gctcgaattc ctgcagccca aatcgcgaaa 1441 ccgaacttaa tgggaggaaa aattaaaaaa gaacagttat gaaaaaaaag attatctcag 1501 ctattttaat gtctacagtg atactttctg ctgcagcccc gttgtcaggt gtttacgctg 1561 acacaaactc agatattgct aaacaagatg cggtcgaccc attgtcaact aaaaaattac 1621 ataaagaacc tgcgacttta attaaagcga ttgatggtga tacggttaaa ttaatgtaca 1681 aaggtcaacc aatgacattc agactattat tggttgatac acctgaaaca aagcatccta 1741 aaaaaggtgt agagaaatat ggtcctgaag caagtgcatt tacgaaaaaa atggtagaaa 1801 atgcaaagaa aattgaagtc gagtttgaca aaggtcaaag aactgataaa tatggacgtg 1861 gcttagcgta tatttatgct gatggaaaaa tggtaaacga agctttagtt cgtcaaggct 1921 tggctaaagt tgcttatgtt tacaaaccta acaatacaca tgaacaacat ttaagaaaaa 1981 gtgaagcaca agcgaaaaaa gagaaattaa atatttggag cgaagacaac gctgattcag 2041 gtcaaatcga tctcgagata tcgctagttc tagattgagg catcaaataa aacgaaaggc 2101 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag 2161 taggacaaat ccgccgctct agctaagcag aaggccatcc tgacggatgg cctttttgcg 2221 tttctacaaa ctcttgttgg ggatccgcct gcagccaagc ttatcgattc tagactcgag 2281 agctctcgag tctagaatcg atacgatttt gaagtggcaa cagataaaaa aaagcagttt 2341 aaaattgttg ctgaactttt aaaacaagca aatacaatca ttgtcgcaac agatagcgac 2401 agagaaggcg aaaacattgc ctggtcgatc attcataaag caaatgcctt ttctaaagat 2461 aaaacgtata aaagactatg gatcaatagt ttagaaaaag atgtgatccg tagcggtttt 2521 caaaatttgc aaccaggaat gaattactat cccttttatc aagaagcgca caaaaagaaa 2581 aacgaaatga tacaccaatc agtgcaaaaa aagatataat gggagataag acggttcgtg 2641 ttcgtgctga cttgcaccat atcataaaaa tcgaaacagc aaagaatggc ggaaacgtaa 2701 aagaagttat ggaaataaga cttagaagca aacttaagag tgtgttgata gtgcagtatc 2761 ttaaaatttt gtataatagg aattgaagtt aaattagatg ctaaaaattt gtaattaaga 2821 aggagtgatt acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa 2881 gtactcaacc aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt 2941 ggaacaggta aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct 3001 attgaattag acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt 3061 gtcactttaa ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa 3121 attgttggga gtattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa 3181 agccatgcgt ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat 3241 attcaccgaa cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag 3301 ctgccagcgg aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc 3361 cgccatacca cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa 3421 tgggtcaatc gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa 3481 cacgccaaag taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat 3541 agttatctat tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa 3601 gttacacgtt actaaaggga atgtagataa attattaggt atactactga cagcttccaa 3661 ggagctaaag aggtccctag cgcttagaat cgctttagga aacacgatcc agtccaataa 3721 tcgtcgataa aaacttttga aaaaggttgg tgaaattacc tacttttgga ataatcacaa 3781 atcacaagtg attaatcaca aatcacaagt gattaatcac ttgtttatta agatattaaa 3841 agctataatt taaataaagc gtgaatttta ttacacaaaa agagggggga gaaacttgga 3901 actagcattt agagaaagct taaaaaagat gagaggtacc aaatcaaaag aaaaattctc 3961 ccaagaatta gaaatgagta gatcaaatta ttcacgaata gaatcaggaa aatcagatcc 4021 aaccataaaa acactagaac aaattgcaaa gttaactaac tcaacgctag tagtggattt 4081 aatcccaaat gagccaacag aaccagaacc agaaacagaa tcagaacaag taacattgga 4141 tttagaaatg gaagaagaaa aaagcaatga cttcgtgtga ataatgcacg aaatcgttgc 4201 ttattttttt taaaagcggt atactagata taacgaaaca acgaactgaa tagaaacgaa 4261 aaaagagcca tgacacattt ataaaatgtt tgacgacatt ttataaatgc atagcccgat 4321 aagattgcca aaccaacgct tatcagttag tcagatgaac tcttccctcg taagaagtta 4381 tttaattaac tttgtttgaa gacggtatat aaccgtacta tcattatata gggaaatcag 4441 agagttttca agtatctaag ctactgaatt taagaattgt taagcaatca atcggaaatc 4501 gtttgattgc tttttttgta ttcatttata gaaggtggag tttgtatgaa tcatgatgaa 4561 tgtaaaactt atataaaaaa tagtttattg gagataagaa aattagcaaa tatctataca 4621 ctagaaacgt ttaagaaaga gttagaaaag agaaatatct acttagaaac aaaatcagat 4681 aagtattttt cttcggaggg ggaagattat atatataagt taatagaaaa taacaaaata 4741 atttattcga ttagtggaaa aaaattgact tataaaggaa aaaaatcttt ttcaaaacat 4801 gcaatattga aacagttgaa tgaaaaagca aaccaagtta attaaacaac ctattttata 4861 ggatttatag gaaaggagaa cagctgaatg aatatccctt ttgttgtaga aactgtgctt 4921 catgacggct tgttaaagta caaatttaaa aatagtaaaa ttcgctcaat cactaccaag 4981 ccaggtaaaa gcaaaggggc tatttttgcg tatcgctcaa aatcaagcat gattggcggt 5041 cgtggtgttg ttctgacttc cgaggaagcg attcaagaaa atcaagatac atttacacat 5101 tggacaccca acgtttatcg ttatggaacg tatgcagacg aaaaccgttc atacacgaaa 5161 ggacattctg aaaacaattt aagacaaatc aataccttct ttattgattt tgatattcac 5221 acggcaaaag aaactatttc agcaagcgat attttaacaa ccgctattga tttaggtttt 5281 atgcctacta tgattatcaa atctgataaa ggttatcaag catattttgt tttagaaacg 5341 ccagtctatg tgacttcaaa atcagaattt aaatctgtca aagcagccaa aataatttcg 5401 caaaatatcc gagaatattt tggaaagtct ttgccagttg atctaacgtg taatcatttt 5461 ggtattgctc gcataccaag aacggacaat gtagaatttt ttgatcctaa ttaccgttat 5521 tctttcaaag aatggcaaga ttggtctttc aaacaaacag ataataaggg ctttactcgt 5581 tcaagtctaa cggttttaag cggtacagaa ggcaaaaaac aagtagatga accctggttt 5641 aatctcttat tgcacgaaac gaaattttca ggagaaaagg gtttaatagg gcgtaataac 5701 gtcatgttta ccctctcttt agcctacttt agttcaggct attcaatcga aacgtgcgaa 5761 tataatatgt ttgagtttaa taatcgatta gatcaaccct tagaagaaaa agaagtaatc 5821 aaaattgtta gaagtgccta ttcagaaaac tatcaagggg ctaataggga atacattacc 5881 attctttgca aagcttgggt atcaagtgat ttaaccagta aagatttatt tgtccgtcaa 5941 gggtggttta aattcaagaa aaaaagaagc gaacgtcaac gtgttcattt gtcagaatgg 6001 aaagaagatt taatggctta tattagcgaa aaaagcgatg tatacaagcc ttatttagtg 6061 acgaccaaaa aagagattag agaagtgcta ggcattcctg aacggacatt agataaattg 6121 ctgaaggtac tgaaggcgaa tcaggaaatt ttctttaaga ttaaaccagg aagaaatggt 6181 ggcattcaac ttgctagtgt taaatcattg ttgctatcga tcattaaagt aaaaaaagaa 6241 gaaaaagaaa gctatataaa ggcgctgaca aattcttttg acttagagca tacattcatt 6301 caagagactt taaacaagct agcagaacgc cctaaaacgg acacacaact cgatttgttt 6361 agctatgata caggctgaaa ataaaacccg cactatgcca ttacatttat atctatgata 6421 cgtgtttgtt ttttctttgc tgtttagcga atgattagca gaaatataca gagtaagatt 6481 ttaattaatt attaggggga gaaggagaga gtagcccgaa aacttttagt tggcttggac 6541 tgaacgaagt gagggaaagg ctactaaaac gtcgaggggc agtgagagcg aagcgaacac 6601 ttgatttttt aattttctat cttttatagg tcattagagt atacttattt gtcctataaa 6661 ctatttagca gcataataga tttattgaat aggtcattta agttgagcat attagaggag 6721 gaaaatcttg gagaaatatt tgaagaaccc gattacatgg attggattag ttcttgtggt 6781 tacgtggttt ttaactaaaa gtagtgaatt tttgattttt ggtgtgtgtg tcttgttgtt 6841 agtatttgct agtcaaagtg attaaataga attcactggc cgtcgtttta caacgtcgtg 6901 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca 6961 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga 7021 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 7081 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac 7141 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 7201 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 7261 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 7321 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 7381 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 7441 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 7501 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 7561 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 7621 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 7681 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 7741 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 7801 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 7861 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 7921 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 7981 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 8041 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 8101 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 8161 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 8221 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 8281 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 8341 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 8401 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 8461 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 8521 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 8581 atcaagagct accaactctt tttcc //