LOCUS AF025843 4079 bp DNA circular SYN 03-FEB-2000 DEFINITION Co-reporter vector pRL-CMV, complete sequence. ACCESSION AF025843 VERSION AF025843.2 KEYWORDS . SOURCE Co-reporter vector pRL-CMV ORGANISM Co-reporter vector pRL-CMV other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4079) AUTHORS Sherf,B., Navarro,S. and Wood,K.V. TITLE Dual-Luciferase(TM) Reporter Assay: An Advanced Co-reporter Technology JOURNAL Promega Notes 57, 2-5 (1996) REFERENCE 2 (bases 1 to 4079) AUTHORS Sherf,B., Navarro,S. and Wood,K.V. TITLE Direct Submission JOURNAL Submitted (20-SEP-1997) Production, Promega Co., 5445 East Cheryl Parkway, Madison, WI 53711, USA REFERENCE 3 (bases 1 to 4079) AUTHORS Grooms,K. TITLE Direct Submission JOURNAL Submitted (17-DEC-1999) Technical Writing, Promega Co., 2800 Woods Hollow Rd., Madison, WI 53711, USA REMARK Sequence update by submitter COMMENT On Dec 17, 1999 this sequence version replaced AF025843.1. FEATURES Location/Qualifiers source 1..4079 /organism="Co-reporter vector pRL-CMV" /mol_type="genomic DNA" /db_xref="taxon:68403" regulatory 7..803 /regulatory_class="promoter" /note="CMV enhancer and immediate-early promoter" intron 860..996 /note="chimeric intron" regulatory 1040..1058 /regulatory_class="promoter" /note="T7 promoter (17 to +2)" misc_feature 1057 /note="T7 promoter transcription start site" gene 1068..2003 /gene="Rluc" CDS 1068..2003 /gene="Rluc" /note="cDNA cloned from the anthozoan coelenterate Renilla reniformis (sea pansy)" /codon_start=1 /transl_table=11 /product="Renilla luciferase" /protein_id="AAB82573.1" /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" regulatory 2045..2246 /regulatory_class="polyA_signal_sequence" /note="SV40 late poly(A) signal" gene 2393..3253 /gene="ampR" CDS 2393..3253 /gene="ampR" /function="ampicillin resistance" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="AAB82574.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" ORIGIN 1 agatcttcaa tattggccat tagccatatt attcattggt tatatagcat aaatcaatat 61 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc 121 atgtccaata tgaccgccat gttggcattg attattgact agttattaat agtaatcaat 181 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 241 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 301 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 361 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt 421 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc 481 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca 541 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat 601 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa 661 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 721 cagagctcgt ttagtgaacc gtcagatcac tagaagcttt attgcggtag tttatcacag 781 ttaaattgct aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagaag 841 ttggtcgtga ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca 901 atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg 961 tcttactgac atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag 1021 ctcttaaggc tagagtactt aatacgactc actataggct agccaccatg acttcgaaag 1081 tttatgatcc agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta 1141 aacaaatgaa tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa 1201 atgctgttat ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc 1261 cacatattga gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag 1321 gcaaatctgg taatggttct tataggttac ttgatcatta caaatatctt actgcatggt 1381 ttgaacttct taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt 1441 tggcatttca ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa 1501 gtgtagtaga tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt 1561 tgatcaaatc tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca 1621 tgttgccatc aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac 1681 cattcaaaga gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt 1741 tagtaaaagg tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac 1801 gtgcaagtga tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg 1861 ctattgttga aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc 1921 atttttcgca agaagatgca cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc 1981 gagttctcaa aaatgaacaa taattctaga gcggccgctt cgagcagaca tgataagata 2041 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga 2101 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 2161 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag 2221 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccaggtggca cttttcgggg 2281 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 2341 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 2401 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 2461 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 2521 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 2581 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 2641 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 2701 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 2761 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 2821 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 2881 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 2941 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 3001 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 3061 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 3121 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 3181 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 3241 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 3301 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 3361 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 3421 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 3481 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 3541 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 3601 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 3661 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 3721 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 3781 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 3841 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 3901 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 3961 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 4021 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tggctcgac //