ID pDoubleEx standard; circular DNA; ; 5716 BP. DT 10-MAY-2009 CC This file is created by Vector NTI CC VNTAUTHORNAME | HonorGene: http://www.honorgene.com CC VNTAUTHORTEL | 15574926881 | CC VNTAUTHOREM L| 12695920@qq.com | FH Key Location/Qualifiers FH FT rep_origin 4991..5634 FT /vntifkey="33" FT /label=pUC\ori FT rep_origin 2857..3087 FT /vntifkey="33" FT /label=f1\ori FT promoter 3149..3184 FT /vntifkey="30" FT /label=K\pro FT rep_origin 3428..3563 FT /vntifkey="33" FT /label=SV40\ori FT promoter 3261..3529 FT /vntifkey="29" FT /label=SV40\pro FT CDS 3612..4406 FT /vntifkey="4" FT /label=Kan+/Neo+ FT polyA_signal 4642..4660 FT /vntifkey="25" FT /label=HSV\TK\PolyA FT polyA_site 2226..2450 FT /invitrogen="1870000" FT /vntifkey="26" FT /label=BGH\PolyA FT primer complement(2220..2237) FT /invitrogen="1910000" FT /vntifkey="27" FT /label=BGH\reverse\primer FT promoter complement(378..1544) FT /vntifkey="29" FT /label=hEF-1¦Á\promoter FT polyA_signal complement(219..321) FT /vntifkey="25" FT /label=SV40\PolyA FT primer complement(421..439) FT /vntifkey="27" FT /label=hEF1¦Á-f FT promoter 1554..2141 FT /vntifkey="29" FT /label=CMV\promoter FT primer 2091..2111 FT /vntifkey="27" FT /label=CMV\forward\primer SQ Sequence 5716 BP; 1337 A; 1573 C; 1513 G; 1293 t; tagttattag ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa 60 caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctttaaa 120 ggaaccaatt cagtcgataa ctataacggt cctaaggtag cgactagtcc atttaaatga 180 taacgcgtcg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 240 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 300 caatgtatct tatcatgtct gaagcttggg gtcgacgcta gatcttcggt accttcgaag 360 cggccgcgac cacgtgttca cgacacctga aatggaagaa aaaaactttg aaccactgtc 420 tgaggcttga gaatgaacca agatccaaac tcaaaaaggg caaattccaa cgagaattac 480 atcaagtgcc aagctggcct aacttcagtc tccacccact cagtgtgggg aaactccatc 540 gcataaaacc cctcccccca acctaaagac gacgtactcc aaaagctcca gaactaatcg 600 aggtgcctgg acggcgcccg gtactccgtg gagtcacatg aagcgacggc tgaggacgga 660 aaggcccttt tcctttgtgt gggtgactca cccgcccgct ctcccgagcg ccgcgtcctc 720 cattttgagc cccctggagc agggccggga agcggccatc tttccgctca cgcaactggt 780 gccgaccggg ccagccttgc cgcccagggc ggggcgatac acggcggcgc gaggccaggc 840 accagagcag gccggccagc ttgagactac ccccgtccga ttctcggtgg ccgcgctcgc 900 aggccccgcc tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc ggccgcgggc 960 ccaaaaaccg aaataccagt gtgcagatcc tggcccgcat ttacaagact atcttgccag 1020 aaaaaaagcg tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt atcgaaagca 1080 gcgagacagg cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg cccaggccag 1140 gcctcaactc aagcacgagg cgaaggggct cctaaagcgc aaggcccgcc cctggctcca 1200 gctcgggatc aagaatcacg tactggagcc aggtggaagt aattcaaggc acgcaagggc 1260 cataacccgt aaagaggcca ggcccgcggg aaccacacac ggcacttacc tgtgttctgg 1320 cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata tacggttctc 1380 ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag tttaccccgc 1440 gccaccttct ctaggcaccg gttcaattgc cgacccctcc ccccaacttc tcggggactg 1500 tgggcgatgt gcgctctgcc cactgacggg caccggagcc tcacgaagct agcgttgaca 1560 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata 1620 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 1680 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 1740 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 1800 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 1860 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 1920 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 1980 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 2040 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 2100 cggtaggcgt gtacggtggg aggtctatat aagcagagct cggatcccgg aattctgcag 2160 atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc 2220 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 2280 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca 2340 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 2400 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc 2460 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag 2520 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 2580 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 2640 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 2700 aaaacttgat tagggtgatg gttcacgtag tgtctagacc cagctttctt gtacaaagtt 2760 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa 2820 aataaaatca ttatttgcca tccagctgat atctacgtga accatcaccc taatcaagtt 2880 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta 2940 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag 3000 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg 3060 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa 3120 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 3180 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct 3240 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 3300 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 3360 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 3420 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 3480 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 3540 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag 3600 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 3660 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 3720 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 3780 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 3840 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 3900 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 3960 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 4020 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 4080 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga 4140 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 4200 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 4260 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 4320 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 4380 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc 4440 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg 4500 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 4560 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga 4620 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt 4680 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc 4740 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg 4800 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta 4860 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 4920 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 4980 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 5040 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 5100 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 5160 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 5220 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 5280 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 5340 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 5400 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 5460 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 5520 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 5580 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 5640 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 5700 tattaccgcc atgcat 5716 //