LOCUS X65306 3197 bp DNA circular SYN 16-JUL-1999 DEFINITION Cloning vector pGEM-3Zf(+). ACCESSION X65306 VERSION X65306.2 KEYWORDS cloning vector; multiple cloning site; phage f1 region; promoter. SOURCE Cloning vector pGEM-3Zf(+) ORGANISM Cloning vector pGEM-3Zf(+) other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3197) AUTHORS Solomon,L.R., Massom,L.R. and Jarrett,H.W. TITLE Enzymatic syntheses of DNA-silicas using DNA polymerase JOURNAL Anal. Biochem. 203 (1), 58-69 (1992) PUBMED 1326235 REFERENCE 2 AUTHORS Technical,Services. TITLE Direct Submission JOURNAL Submitted (23-MAR-1992) Technical Services, Promega Corporation, 2800 Woods Hollow Road, Madison, Wi 53711-5399, USA REMARK revised by [3] REFERENCE 3 AUTHORS Technical,Services. TITLE Direct Submission JOURNAL Submitted (28-MAY-1993) Technical Services, Promega Corporation, 2800 Woods Hollow Road, Madison, Wi 53711-5399, USA REMARK revised by [4] REFERENCE 4 (bases 1 to 3197) AUTHORS Kenefick,K. TITLE Direct Submission JOURNAL Submitted (14-JUL-1999) Kenefick K.,Technical Services, Promega Corporation, 2800 Woods Hollow Road, Madison, Wi 53711-5399, USA COMMENT On Jul 19, 1999 this sequence version replaced X65306.1. See X65300-X65335 for related vector sequences This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information: Order or Technical 800-356-9526 In Wisconsin 800-356-9526 Outside U.S. 608-274-4330. FEATURES Location/Qualifiers source 1..3197 /organism="Cloning vector pGEM-3Zf(+)" /mol_type="other DNA" /db_xref="taxon:90112" misc_feature 1 /note="T7 transcription initiation site" regulatory join(3181..3197,3) /regulatory_class="promoter" /note="T7 promoter" misc_feature 5..61 /note="multiple cloning sites" regulatory 67..86 /regulatory_class="promoter" /note="SP6 promoter" misc_feature 69 /note="SP6 transcription initiation site" misc_feature 94..323 /note="lac operon sequence" gene complement(106..108) /gene="lacZ" misc_feature complement(106..108) /gene="lacZ" /note="lacZ start codon" gene complement(1265..2125) /gene="bla" CDS complement(1265..2125) /gene="bla" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="CAA46398.2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" misc_feature 2562..3017 /note="phage f1 region" misc_feature 3018..3178 /note="lac operon sequence" ORIGIN 1 gggcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct 61 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg 121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 181 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 241 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 301 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 421 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 481 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 1021 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 1081 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 1141 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 1201 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 1261 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 1321 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 1381 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 1441 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 1501 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 1561 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 1621 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 1681 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 1741 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 1801 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 1861 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 1921 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 1981 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 2041 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 2101 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 2161 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 2221 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 2281 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 2341 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 2401 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 2461 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 2521 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat 2581 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 2641 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 2701 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 2761 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 2821 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 2881 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 2941 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 3001 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg 3061 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta 3121 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg 3181 taatacgact cactata //