LOCUS       pNZ8048                3349 bp ds-DNA     circular SYN 14-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3349)
AUTHOR Name | HonorGene: http://www.honorgene.com
AUTHOR Tel | 15574926881 |
AUTHOR Email | 12695920@qq.com |

FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        5..188
                     /label=PnisA
     terminator      393..445
                     /label=T
     CDS             971..1180
                     /codon_start=1
                     /label=repC
                     /translation="MGGKEANFASVLRPPIKCRVPIFVPKTLYPNWLKGLRGFSIANES
                     PTFSPTFFINLYLSSFIVVFMITK"
     CDS             1448..2146
                     /codon_start=1
                     /label=repA
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     CDS             2605..3255
                     /codon_start=1
                     /label=Cm
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
ORIGIN
        1 agatctagtc ttataactat actgacaata gaaacattaa caaatctaaa acagtcttaa
       61 ttctatcttg agaaagtatt ggtaataata ttattgtcga taacgcgagc ataataaacg
      121 gctctgatta aattctgaag tttgttagat acaatgattt cgttcgaagg aactacaaaa
      181 taaattataa ggaggcactc accatgggta ctgcaggcat gcggtaccac tagttctaga
      241 gagctcaagc tttctttgaa ccaaaattag aaaaccaagg cttgaaacgt tcaattgaaa
      301 tggcaattaa acaaattaca gcacgtgttg ctttgattga tagccaaaaa gcagcagttg
      361 ataaagcaat tactgatatt gctgaaaaat tgtaatttat aaataaaaat caccttttag
      421 aggtggtttt tttatttata aattattcgt ttgatttcgc tttcgataga acaatcaaag
      481 cgagaataag gaagataaat cccataaggg cgggagcaga atgtccgaga ctaatgtaaa
      541 tttgtccacc aattaaagga ccgataacgc gagcttctcg agtgcatatt ttcggcaatc
      601 ttctcaatga gatgctcttc agcatgttca atgatgtcga ttttttatta aaacgtctca
      661 aaatcgtttc tgagacgttt tagcgtttat ttcgtttagt tatcggcata atcgttaaaa
      721 caggcgttat cgtagcgtaa aagcccttga gcgtagcgtg ctttgcagcg aagatgttgt
      781 ctgttagatt atgaaagccg atgactgaat gaaataataa gcgcagcgtc cttctatttc
      841 ggttggagga ggctcaaggg agtttgaggg aatgaaattc cctcatgggt ttgattttaa
      901 aaattgcttg caattttgcc gagcggtagc gctggaaaat ttttgaaaaa aatttggaat
      961 ttggaaaaaa atggggggaa aggaagcgaa ttttgcttcc gtactacgac cccccattaa
     1021 gtgccgagtg ccaatttttg tgccaaaaac gctctatccc aactggctca agggtttgag
     1081 gggtttttca atcgccaacg aatcgccaac gttttcgcca acgtttttta taaatctata
     1141 tttaagtagc tttattgttg tttttatgat tacaaagtga tacactaatt ttataaaatt
     1201 atttgattgg agttttttaa atggtgattt cagaatcgaa aaaaagagtt atgatttctc
     1261 tgacaaaaga gcaagataaa aaattaacag atatggcgaa acaaaaaggt ttttcaaaat
     1321 ctgcggttgc ggcgttagct atagaagaat atgcaagaaa ggaatcagaa caaaaaaaat
     1381 aagcgaaagc tcgcgttttt agaaggatac gagttttcgc tacttgtttt tgataaggta
     1441 atatatcatg gctattaaaa atactaaagc tagaaatttt ggatttttat tatatcctga
     1501 ctcaattcct aatgattgga aagaaaaatt agagagtttg ggcgtatcta tggctgtcag
     1561 tcctttacac gatatggacg aaaaaaaaga taaagataca tggaatagta gtgatgttat
     1621 acgaaatgga aagcactata aaaaaccaca ctatcacgtt atatatattg cacgaaatcc
     1681 tgtaacaata gaaagcgtta ggaacaagat taagcgaaaa ttggggaata gttcagttgc
     1741 tcatgttgag atacttgatt atatcaaagg ttcatatgaa tatttgactc atgaatcaaa
     1801 ggacgctatt gctaagaata aacatatata cgacaaaaaa gatattttga acattaatga
     1861 ttttgatatt gaccgctata taacacttga tgaaagccaa aaaagagaat tgaagaattt
     1921 acttttagat atagtggatg actataattt ggtaaataca aaagatttaa tggcttttat
     1981 tcgccttagg ggagcggagt ttggaatttt aaatacgaat gatgtaaaag atattgtttc
     2041 aacaaactct agcgccttta gattatggtt tgagggcaat tatcagtgtg gatatagagc
     2101 aagttatgca aaggttcttg atgctgaaac gggggaaata aaatgacaaa caaagaaaaa
     2161 gagttatttg ctgaaaatga ggaattaaaa aaagaaatta aggacttaaa agagcgtatt
     2221 gaaagataca gagaaatgga agttgaatta agtacaacaa tagatttatt gagaggaggg
     2281 attattgaat aaataaaagc ccccctgacg aaagtcgacg gcaatagtta cccttattat
     2341 caagataaga aagaaaagga tttttcgcta cgctcaaatc ctttaaaaaa acacaaaaga
     2401 ccacattttt taatgtggtc tttattcttc aactaaagca cccattagtt caacaaacga
     2461 aaattggata aagtgggata tttttaaaat atatatttat gttacagtaa tattgacttt
     2521 taaaaaagga ttgattctaa tgaagaaagc agacaagtaa gcctcctaaa ttcactttag
     2581 ataaaaattt aggaggcata tcaaatgaac tttaataaaa ttgatttaga caattggaag
     2641 agaaaagaga tatttaatca ttatttgaac caacaaacga cttttagtat aaccacagaa
     2701 attgatatta gtgttttata ccgaaacata aaacaagaag gatataaatt ttaccctgca
     2761 tttattttct tagtgacaag ggtgataaac tcaaatacag cttttagaac tggttacaat
     2821 agcgacggag agttaggtta ttgggataag ttagagccac tttatacaat ttttgatggt
     2881 gtatctaaaa cattctctgg tatttggact cctgtaaaga atgacttcaa agagttttat
     2941 gatttatacc tttctgatgt agagaaatat aatggttcgg ggaaattgtt tcccaaaaca
     3001 cctatacctg aaaatgcttt ttctctttct attattcctt ggacttcatt tactgggttt
     3061 aacttaaata tcaataataa tagtaattac cttctaccca ttattacagc aggaaaattc
     3121 attaataaag gtaattcaat atatttaccg ctatctttac aggtacatca ttctgtttgt
     3181 gatggttatc atgcaggatt gtttatgaac tctattcagg aattgtcaga taggcctaat
     3241 gactggcttt tataatatga gataatgccg actgtacttt ttacagtcgg ttttctaatg
     3301 tcactaacct gccccgttag ttgaagaagg tttttatatt acagctcca
//