LOCUS pKC1139 6573 bp DNA circular 14-MAR-2012 FEATURES Location/Qualifiers CDS complement(167..508) /vntifkey="4" /label=lacZa CDS 1730..2503 /vntifkey="4" /label=aac(3)IV primer complement(509..525) /vntifkey="27" /label=sp6 primer complement(528..547) /vntifkey="27" /label=seq-152-anti misc_feature 765..934 /vntifkey="21" /label=ori-PUC18 misc_feature 6050..6161 /vntifkey="21" /label=oriT CDS complement(3443..4885) /vntifkey="4" /label=Rep-pSG5 promoter complement(4855..5273) /vntifkey="30" /label=promoter promoter 1662..1711 /vntifkey="30" /label=promoter BASE COUNT 1180 a 2002 c 2082 g 1309 t ORIGIN 1 gatctacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc 61 agctctcgca gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg 121 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt gatcggcact 181 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattt atgcggtgtg 241 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 301 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 361 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 421 gttgtaaaac gacggccagt gccaagcttg ggctgcaggt cgactctaga ggatccgcgg 481 ccgcgcgcga tatcgaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc 541 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 601 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 661 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 721 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 781 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 841 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 901 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 961 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 1021 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 1081 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 1141 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 1201 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 1261 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 1321 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 1381 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 1441 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 1501 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 1561 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg gttcatgtgc 1621 agctccatca gcaaaagggg atgataagtt tatcaccacc gactatttgc aacagtgccg 1681 ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag tgcaatgtcg tgcaatacga 1741 atggcgaaaa gccgagctca tcggtcagct tctcaacctt ggggttaccc ccggcggtgt 1801 gctgctggtc cacagctcct tccgtagcgt ccggcccctc gaagatgggc cacttggact 1861 gatcgaggcc ctgcgtgctg cgctgggtcc gggagggacg ctcgtcatgc cctcgtggtc 1921 aggtctggac gacgagccgt tcgatcctgc cacgtcgccc gttacaccgg accttggagt 1981 tgtctctgac acattctggc gcctgccaaa tgtaaagcgc agcgcccatc catttgcctt 2041 tgcggcagcg gggccacagg cagagcagat catctctgat ccattgcccc tgccacctca 2101 ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat gggcaggtac ttctcctcgg 2161 cgtgggacac gatgccaaca cgacgctgca tcttgccgag ttgatggcaa aggttcccta 2221 tggggtgccg agacactgca ccattcttca ggatggcaag ttggtacgcg tcgattatct 2281 cgagaatgac cactgctgtg agcgctttgc cttggcggac aggtggctca aggagaagag 2341 ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg ttgatccgct cccgcgacat 2401 tgtggcgaca gccctgggtc aactgggccg agatccgttg atcttcctgc atccgccaga 2461 ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt ggctgagctc atgagcggag 2521 aacgagatga cgttggaggg gcaaggtcgc gctgattgct ggggcaacac gtggagcgga 2581 tcggggattg tctttcttca gctcgctgat gatatgctga cgctcaatgc cgtttggcct 2641 ccgactaacg aaaatcccgc atttggacgg ctgatccgat tggcacggcg gacggcgaat 2701 ggcggagcag acgctcgtcc gggggcaatg agatatgaaa aagcctgaac tcaccgcgac 2761 gtatcgatgt cgaggttcct caggggagcc accccagaga agccctcgga gctgagcgga 2821 gctatttcca aagccatgcc agctagagac agtgcacacc gccaagcatc tgcaaaaccc 2881 tcgcctggag ggaaagtgca atgtacgtac gctggtttcc ctccagaaag ggattctgcg 2941 gcttctcaac tttggaagaa gaggcggaac gaactgctgt ttcagcctac tcatgtgagg 3001 aggctggtcc tttaccctga tgcccgaggg catcagggta aaggaccagc ctcgccaact 3061 aggagcgcta ccaaggcgaa cacaacccag gacttggtag aacccttcgc cggagaagtt 3121 cagactcatc ggcatgagca cccctcgccg cgcactcggc accggtccgt cgaccaccac 3181 caggacgtcg ttgtcgacgt cggccccgcg gctcctgccc gccgaacgcg tcgtcgtcga 3241 cggcctggtg ctcatcgacg agcacccgga gccaggtgaa aagcgccggc ggacgctcgg 3301 actgggcgcg ggattccagc agtaacccag gtccgccggc cacctcacgg caggcagacc 3361 ctcggctttc gcgccgggac cgccatgaga ccgccacccg gatgtccggg gtggcggtct 3421 catgggcggt ccctcagcgg ccctacgcga ccgctgtgtc gacgcggagg cagtctccgg 3481 ggtgctggcc ccagggctgg agtaccgggg cgagcttgcc cttgcagcgg cggcacacgg 3541 gcgacgcggc gccgggcggg ggagtcttga cgtcgacggt gaccggcttc ggcttcaggc 3601 gcttccgaag gctgatcgtc gggcggatcg cctccttctt cggctgacgc actcggtcga 3661 gcgaagcggc cagctcctcc gtgcgggcct cgttggcctt ccggcgcgcc tcgcggaaag 3721 cagcttcctc ctcggacatg acctcgaacc tcatctggtc agcgtcaagg tcgcccggcg 3781 cggccggcgc ttccggggcg ggcgggtcca ggacgtcctt gccccacacc aggccccagg 3841 actcgacgag ccgccggacg cccggtaggc cgtacgtctc ggcgaccttg atgaggtcga 3901 ggcgacgtcc ggcgacgcgg gcgatgtatc ggtaccagat gtaggccggg atgaccgcga 3961 tggcgaccag gccctcggtg tcgtcggtga tctcctcctc ggtgcggacg tcctgctgga 4021 tgccgagttc cttgatcagc cggttcaggt tctgcgaccg gtagtgcttg cggacctgga 4081 agacgccgaa ctcgcgctcg cggtacttct cgacgaacgg gccgggccga cgaagccgct 4141 gcagctcggc ggccgccgcg tcgcccaggt cgagcggtcc catgcggtcg tcgccgcgac 4201 cggccttgaa gttctgtccg gccagctcca ggccgatctt ggcgacgccg cccttggtct 4261 tgtcgccgtc cttgtagagg tagcgggcct gcttgcccgc atcgccgtca gcggcgtccg 4321 cgccgttgag tgggcgcacg tcggtgccgt ggcccttgcc ctcacaggag caaccgggcc 4381 ggtcgcacgt ctcgctgacg gtgtagccgc ccgcggattc gaccccggcg gcccaggctc 4441 cggcgagtgc gtcgcggaac gcggcctggg cgtccgggcc gagcacctcg cgggtgaccc 4501 agagcgtgtg ccagtgcagg tgccagccgg agccccagcc gaaggtgtcc tcgaaggccc 4561 gctcgtagcc gatgatcccg aagtcgtcgc gcatcgtgcg ccagcggcgg ccggacgagc 4621 cgtacgcgcc cttccagccg tcgtgcaaga ccgcgaccag gccgtgccgc attcccttgc 4681 ggacggtgcc gaacgccatg cgctcgaagt ggcgcaacgt gttcgtgcca aggtgcagcc 4741 cgtacccggc gtccgcgaga ccgtcggcgg cgagctgcac gttcgagccc cgtacggcca 4801 ggatgcggct catgcaccac gggcaggtgt ggacgttgtt gcagcggcac gtgttgcccc 4861 acgtcgcctc gcccggcttc cacatcagct cggccgtccc ggcagtgagc cgggtcccgc 4921 agcccttgaa cgcctcgttc agcgacaccg tctggtgccg gtcccgccgg gcgaaccgct 4981 cgtcgcgcgg gtcctcccgc ccggctgtcg cggcaccctc gtttggggta gaacccgttc 5041 cagttacagc gctctgacct gcagtggacg gagattttcc cttactacta aagcccgcgt 5101 ccggattacc cgctgtagtc gtgcttgcta cgctgcgtga ctggtccgca atgagacgct 5161 ttgcgcgctt tcggcaggcg tccgagcagt agattttggg gcgcttcccg gggatgtgga 5221 cgatcggggt gccgcagtgg cacttcggtc cggcggggcg cggtggtgtc gacgcgctgt 5281 tctctcgtac gctcgtcaca gagcaaacgt cctcactcgg catgctgcgc cggttcgggg 5341 gcggcgagcc cgggaggcca atcccgggct cgtgccattt ctgggtcctg ttgatcctgg 5401 cattggtgtg gccgttcatt gcccctgctc gctcctgacg cgccgataga cgtccgatac 5461 gcccggtgct ggtgggattt gataggtcgg aagaagcccc gcccgggcct gggcggggct 5521 tcctgtgcgt caggacctcc tcgtcgtgag cctcttcggc ctatggacgg agtgacctcg 5581 tgatccgtta cagccgcgcg cgctcgcgta gagcggtctc atcagttcca cgaacggtcc 5641 tcttcgcaga tcagggcgtt ggggcggagt ctcaccaagg actacgtctg ctggcgattt 5701 ccgttacacc ccgggcggtg gccggcgcac acgcgcgccc gcgttgggca gtgcagaaag 5761 tgcagaaacc taggcgctga tggtccaggt ccacggttcg tcgtcggcgg cggcgcgggc 5821 ggcggcgtcg gccagggcgc gggcgagacc ggctacggcg ggcttgatgc gccggttgcg 5881 ggcgaccttg agcagctagt atgcaggtcg acggatcttt tccgctgcat aaccctgctt 5941 cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt 6001 ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat 6061 aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct 6121 ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gcatgagcct gtcggcctac 6181 ctgctggccg tcggccaggg ctacaaaatc acgggcgtcg tggactatga gcacgtccgc 6241 gagctggccc gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa actctggctc 6301 accgacgacc cgcgcacggc gcggttcggt gatgccacga tcctcgccct gctggcgaag 6361 atcgaagaga agcaggacga gcttggcaag gtcatgatgg gcgtggtccg cccgagggca 6421 gagccatgac ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt 6481 ccccatgcgc tccatcaaga agagcgactt cgcggagctg gtgaagtaca tcaccgacga 6541 gcaaggcaag accgatcccc ggggacctgc ata //