LOCUS AF406991 9663 bp DNA linear SYN 13-NOV-2003 DEFINITION Tobacco rattle virus RNA2-based VIGS vector pTRV2, complete sequence. ACCESSION AF406991 VERSION AF406991.1 GI:21389151 KEYWORDS . SOURCE Tobacco rattle virus RNA2-based VIGS vector pTRV2 (pYL156) ORGANISM Tobacco rattle virus RNA2-based VIGS vector pTRV2 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9663) AUTHORS Liu,Y., Schiff,M., Marathe,R. and Dinesh-Kumar,S.P. TITLE Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus JOURNAL Plant J. 30 (4), 415-429 (2002) PUBMED 12028572 REFERENCE 2 (bases 1 to 9663) AUTHORS Liu,Y. TITLE Direct Submission JOURNAL Submitted (08-AUG-2001) MCDB, Yale University, 165 Prospect Street, New Haven, CT 06520, USA FEATURES Location/Qualifiers source 1..9663 /organism="Tobacco rattle virus RNA2-based VIGS vector pTRV2" /mol_type="other DNA" /db_xref="taxon:188057" /note="Tobacco rattle virus RNA2-based T-DNA vector synonym: pYL156" misc_feature 1..1639 /note="TRV strain ppk20 RNA2 5'-sequence" misc_feature 1640..1708 /note="multiple cloning site region" misc_feature 1709..2103 /note="TRV strain ppk20 RNA2 3'-sequence" misc_feature 2104..2154 /note="synthetic sequence" misc_feature 2155..8894 /note="pCambia 0390 sequence" promoter 8895..9663 /note="CaMV 35S promoter from pCASS2" ORIGIN 1 ataaaacatt gcacctatgg tgttgccctg gctggggtat gtcagtgatc gcagtagaat 61 gtactaattg acaagttgga gaatacggta gaacgtcctt atccaacaca gcctttatcc 121 ctctccctga cgaggttttt gtcagtgtaa tatttctttt tgaactatcc agcttagtac 181 cgtacgggaa agtgactggt gtgcttatct ttgaaatgtt actttgggtt tcggttcttt 241 aggttagtaa gaaagcactt gtcttctcat acaaaggaaa acctgagacg tatcgcttac 301 gaaagtagca atgaaagaaa ggtggtggtt ttaatcgcta ccgcaaaaac gatggggtcg 361 ttttaattaa cttctcctac gcaagcgtct aaacggacgt tggggttttg ctagtttctt 421 tagagaaaac tagctaagtc tttaatgtta tcattagaga tggcataaat ataatacttg 481 tgtctgctga taagatcatt ttaatttgga cgattagact tgttgaacta caggttactg 541 aatcacttgc gctaatcaac atgggagata tgtacgatga atcatttgac aagtcgggcg 601 gtcctgctga cttgatggac gattcttggg tggaatcagt ttcgtggaaa gatctgttga 661 agaagttaca cagcataaaa tttgcactac agtctggtag agatgagatc actgggttac 721 tagcggcact gaatagacag tgtccttatt caccatatga gcagtttcca gataagaagg 781 tgtatttcct tttagactca cgggctaaca gtgctcttgg tgtgattcag aacgcttcag 841 cgttcaagag acgagctgat gagaagaatg cagtggcggg tgttacaaat attcctgcga 901 atccaaacac aacggttacg acgaaccaag ggagtactac tactaccaag gcgaacactg 961 gctcgacttt ggaagaagac ttgtacactt attacaaatt cgatgatgcc tctacagctt 1021 tccacaaatc tctaacttcg ttagagaaca tggagttgaa gagttattac cgaaggaact 1081 ttgagaaagt attcgggatt aagtttggtg gagcagctgc tagttcatct gcaccgcctc 1141 cagcgagtgg aggtccgata cgtcctaatc cctagggatt taaggacgtg aactctgttg 1201 agatctctgt gaaattcaga gggtgggtga taccatattc actgatgcca ttagcgacat 1261 ctaaataggg ctaattgtga ctaatttgag ggaatttcct ttaccattga cgtcagtgtc 1321 gttggtagca tttgagtttc gcaatgcacg aattacttag gaagtggctt gacgacacta 1381 atgtgttatt gttagataat ggtttggtgg tcaaggtacg tagtagagtc ccacatattc 1441 gcacgtatga agtaattgga aagttgtcag tttttgataa ttcactggga gatgatacgc 1501 tgtttgaggg aaaagtagag aacgtatttg tttttatgtt caggcggttc ttgtgtgtca 1561 acaaagatgg acattgttac tcaaggaagc acgatgagct ttattattac ggacgagtgg 1621 acttagattc tgtgagtaag gttaccgaat tctctagaag gcctccatgg ggatccggta 1681 ccgagctcac gcgtctcgag gcccgggcat gtcccgaaga cattaaacta cggttcttta 1741 agtagatccg tgtctgaagt tttaggttca atttaaacct acgagattga cattctcgac 1801 tgatcttgat tgatcggtaa gtcttttgta atttaatttt ctttttgatt ttattttaaa 1861 ttgttatctg tttctgtgta tagactgttt gagatcggcg tttggccgac tcattgtctt 1921 accatagggg aacggacttt gtttgtgttg ttattttatt tgtattttat taaaattctc 1981 aacgatctga aaaagcctcg cggctaagag attgttgggg ggtgagtaag tacttttaaa 2041 gtgatgatgg ttacaaaggc aaaaggggta aaacccctcg cctacgtaag cgttattacg 2101 cccgtctgta cttatatcag tacactgacg agtccctaaa ggacgaaacg ggagaacgct 2161 agccaccacc accaccacca cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg 2221 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat 2281 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac 2341 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat 2401 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt 2461 actagatcgg gaattaaact atcagtgttt gacaggatat attggcgggt aaacctaaga 2521 gaaaagagcg tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc 2581 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc 2641 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa 2701 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc 2761 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac 2821 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg 2881 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt 2941 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc 3001 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc 3061 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg 3121 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg 3181 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg 3241 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc 3301 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg 3361 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg 3421 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg 3481 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga 3541 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga 3601 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc 3661 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa 3721 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg 3781 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag 3841 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc 3901 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc 3961 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac 4021 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac 4081 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct 4141 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg 4201 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc 4261 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg 4321 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc 4381 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt 4441 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc 4501 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga 4561 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac 4621 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa 4681 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat 4741 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg 4801 ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc 4861 gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg 4921 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc 4981 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg 5041 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc 5101 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga 5161 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga 5221 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga 5281 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga 5341 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt 5401 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt 5461 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac 5521 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg 5581 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa 5641 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg 5701 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa 5761 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg 5821 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc 5881 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc 5941 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg 6001 tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa 6061 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga 6121 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct 6181 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa 6241 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc 6301 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca 6361 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt 6421 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 6481 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 6541 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 6601 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 6661 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 6721 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 6781 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 6841 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 6901 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 6961 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 7021 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 7081 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 7141 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 7201 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 7261 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 7321 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 7381 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 7441 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 7501 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc 7561 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa 7621 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc 7681 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt 7741 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc 7801 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc 7861 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc 7921 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa 7981 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata 8041 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg 8101 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc 8161 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata 8221 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc 8281 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc 8341 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat 8401 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca 8461 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg 8521 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc 8581 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg 8641 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg 8701 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc 8761 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca 8821 cattgcggac gtttttaatg tactgaatta acgccgaatt aattcctagg ccaccatgtt 8881 gggcccggcg cgccaagctt gcatgcctgc aggtcaacat ggtggagcac gacactctcg 8941 tctactccaa gaatatcaaa gatacagtct cagaagacca gagggctatt gagacttttc 9001 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca 9061 tcgaaaggac agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa 9121 aggctatcgt tcaagatgcc tctaccgaca gtggtcccaa agatggaccc ccacccacga 9181 ggaacatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg 9241 atggtcaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc 9301 tcagaagacc agagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc 9361 ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc 9421 ttctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctaccgac 9481 agtggtccca aagatggacc cccacccacg aggaacatcg tggaaaaaga agacgttcca 9541 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca 9601 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag 9661 agg //