File: pEF1/ mycHis lacZ seq
Mode: Normal              1 -    9226
Table: DNASIS#  Style: Circular  Indication Mode: Actual Cutting Site   

Enzyme Name Sequence             Count Cutting Position
AatI        AGG!CCT                  3    847    890   5969
AatII       GACGT!C                  2    122   2393
Acc65I      G!GTACC                  2   1716   1950
AccI        GT!MKAC                  5    122    156   4534   7153   7160
AccIII      T!CCGGA                  0
AcyI        GR!CGYC                  9    119   1415   2390   3918   4090
                                         6180   6884   6963   8963
AflI        G!GWCC                   5    442   3311   6696   8564   8786
AflII       C!TTAAG                  1    855
AflIII      A!CRYGT                  7    351   1109   3072   3852   4277
                                         4529   7533
AgeI        A!CCGGT                  2    554   4891
AhaII       GR!CGYC                  9    119   1415   2390   3918   4090
                                         6180   6884   6963   8963
AhaIII      TTT!AAA                  6    414    977   4921   8292   8311
                                         9003
Alw44I      G!TGCAC                  4   4115   4230   7847   9093
AlwI        GGATCNNNN!              15    135   1594   1736   3187   3479
                                         4520   4883   6024   6048   6359
                                         6605   6999   8182   8279   9059
AlwI        !NNNNNGATCC             14    138    800   1723   1771   3037
                                         3187   3351   6424   6970   8094
                                         8180   8278   8742   9063
AlwNI       CAGNNN!CTG               5    203   3480   3905   4656   7949
AocI        CC!TNAGG                 1   1994
Aor51HI     AGC!GCT                  1   3605
AosI        TGC!GCA                  3   1912   6282   8648
AosII       GR!CGYC                  9    119   1415   2390   3918   4090
                                         6180   6884   6963   8963
ApaI        GGGCC!C                  1   1093
ApaLI       G!TGCAC                  4   4115   4230   7847   9093
ApoI        R!AATTY                  8    250    979   1565   1751   2266
                                         4774   5626   7072
AquI        C!YCGRG                  9    521    623    809   1324   1441
                                         3108   4551   4835   5991
AscI        GG!CGCGCC                0
AseI        AT!TAAT                  4   5640   7304   7363   8598
AsnI        AT!TAAT                  4   5640   7304   7363   8598
Asp700I     GAANN!NNTTC              3    775   5641   9025
Asp718I     G!GTACC                  2   1716   1950
AspHI       GWGCW!C                 10   1304   1726   3710   4119   4234
                                         6293   6483   7851   9012   9097
AspI        GACN!NNGTC               1   6298
AsuII       TT!CGAA                  1   4850
AvaI        C!YCGRG                  9    521    623    809   1324   1441
                                         3108   4551   4835   5991
AvaII       G!GWCC                   5    442   3311   6696   8564   8786
AvaIII      ATGCA!T                  3   4889   5720   5792
AviII       TGC!GCA                  3   1912   6282   8648
AvrI        !CYCGRG                  9    520    622    808   1323   1440
                                         3107   4550   4834   5990
AvrII       C!CTAGG                  1   5970
AxyI        CC!TNAGG                 1   1994
BalI        TGG!CCA                  1   6262
BamHI       G!GATCC                  1   1728
BanII       GRGCY!C                  9    865   1093   1304   1726   3710
                                         4759   5161   5197   6545
BanIII      AT!CGAT                  1   2594
BbeI        GGCGC!C                  2   1418   6183
BbiII/AcyI  GR!CGYC                  9    119   1415   2390   3918   4090
                                         6180   6884   6963   8963
BbrPI       CAC!GTG                  0
BbsI        !NNNNNNGTCTTC            1   4539
BbsI        GAAGACNN!                2   3287   5132
BbvII       GAAGACNN!                2   3287   5132
BbvII       !NNNNNNGTCTTC            1   4539
BcefI       ACGGCNNNNNNNNNNNN!      14   2256   2352   2730   2808   2988
                                         3027   3165   3254   3516   3537
                                         4250   5416   6606   8035
BcefI       !NNNNNNNNNNNNNGCCGT     10    648    699   1193   1368   1403
                                         2077   2245   2785   2900   6140
BclI        T!GATCA                  3   3116   4930   6021
BfrI        C!TTAAG                  1    855
BglI        GCCNNNN!NGGC             6   1247   1423   1540   1922   4043
                                         8546
BglII       A!GATCT                  2    135   1042
BinI        !NNNNNGATCC             14    138    800   1723   1771   3037
                                         3187   3351   6424   6970   8094
                                         8180   8278   8742   9063
BinI        GGATCNNNN!              15    135   1594   1736   3187   3479
                                         4520   4883   6024   6048   6359
                                         6605   6999   8182   8279   9059
BlnI        C!CTAGG                  1   5970
Bpu1102I    GC!TNAGC                 1   4783
BsaAI       YAC!GTR                  3    796   5443   6484
BsaBI       GATNN!NNATC              2   3101   6039
BsaHI       GR!CGYC                  9    119   1415   2390   3918   4090
                                         6180   6884   6963   8963
BsaI        GGTCTCN!                 0
BsaI        !NNNNNGAGACC             2   1684   8487
BseAI       T!CCGGA                  0
BsePI       !GCGCGC                  2   3267   6576
BsiWI       C!GTACG                  1   4541
BsmAI       !NNNNNGAGAC              5   1514   1684   3337   6022   8487
BsmAI       GTCTCN!                  5     37    950    969   1170   2397
BsmI        NG!CATTC                 1   7102
BsmI        GAATGCN!                 0
BspDI       AT!CGAT                  1   2594
BspEI       T!CCGGA                  0
BspHI       T!CATGA                  2     35   8253
BspMI       ACCTGCNNNN!              3   1000   6448   6900
BspMI       !NNNNNNNNGCAGGT          3   2544   4416   6067
BspMII      T!CCGGA                  0
BsrFI       R!CCGGY                 10    554   1176   3057   4789   4891
                                         5338   6499   6680   6965   8506
BssHII      G!CGCGC                  2   3268   6577
Bst1107I    GTA!TAC                  2   4535   7154
BstBI       TT!CGAA                  1   4850
BstEII      G!GTNACC                 1   4840
BstI        G!GATCC                  1   1728
BstPI       G!GTNACC                 1   4840
BstXI       CCANNNNN!NTGG            3   1747   3990   4607
Bsu36I      CC!TNAGG                 1   1994
CbiI        TT!CGAA                  1   4850
CcrI        C!TCGAG                  2   1441   4835
CelII       GC!TNAGC                 1   4783
Cfr10I      R!CCGGY                 10    554   1176   3057   4789   4891
                                         5338   6499   6680   6965   8506
Cfr9I       C!CCGGG                  1   5991
CfrI        Y!GGCCR                 12   1139   1174   1274   2241   3239
                                         4829   6086   6260   6651   6678
                                         7372   8814
ClaI        AT!CGAT                  1   2594
CpoI        CG!GWCCG                 1   6696
Csp45I      TT!CGAA                  1   4850
CspI        CG!GSCCG                 0
CvnI        CC!TNAGG                 1   1994
DraI        TTT!AAA                  6    414    977   4921   8292   8311
                                         9003
DraII       RG!GNCCY                 3   1089   1126   1365
DraIII      CACNNN!GTG               3   1512   2960   5446
DrdI        GACNNNN!NNGTC            4   3879   5490   6207   7641
DsaI        C!CRYGG                 10    727   1074   1401   2102   3009
                                         3147   3236   3508   5877   6612
EaeI        Y!GGCCR                 12   1139   1174   1274   2241   3239
                                         4829   6086   6260   6651   6678
                                         7372   8814
EagI        C!GGCCG                  2   4829   6086
Eam1105I    GACNNN!NNGTC             1   8426
EarI        !NNNNGAAGAG              4   1871   4865   6524   6734
EarI        CTCTTCN!                 2   7417   9221
Ecl136II    GAG!CTC                  3   1302   1724   3708
EclXI       C!GGCCG                  2   4829   6086
Eco105I     TAC!GTA                  0
Eco31I      GGTCTCN!                 0
Eco31I      !NNNNNGAGACC             2   1684   8487
Eco47I      G!GWCC                   5    442   3311   6696   8564   8786
Eco47III    AGC!GCT                  1   3605
Eco52I      C!GGCCG                  2   4829   6086
Eco81I      CC!TNAGG                 1   1994
EcoNI       CCTNN!NNNAGG             2    565   1293
EcoO109I    RG!GNCCY                 3   1089   1126   1365
EcoO65I     G!GTNACC                 1   4840
EcoRI       G!AATTC                  2   1751   4774
EcoRV       GAT!ATC                  1   2883
EcoT14I     C!CWWGG                  4   1560   5877   5970   6612
EcoT22I     ATGCA!T                  3   4889   5720   5792
EcoT38I     GRGCY!C                  9    865   1093   1304   1726   3710
                                         4759   5161   5197   6545
EheI        GGC!GCC                  2   1416   6181
Esp3I       CGTCTCN!                 1   2397
Esp3I       !NNNNNGAGACG             1   3337
EspI        GC!TNAGC                 1   4783
FbaI        T!GATCA                  3   3116   4930   6021
FokI        GGATGNNNNNNNNN!         12   2382   2872   3001   3289   3732
                                         3745   3781   5161   6047   6504
                                         6529   6985
FokI        !NNNNNNNNNNNNNCATCC     11   1832   2822   2927   3584   3952
                                         4376   5356   5821   8392   8573
                                         8860
FspI        TGC!GCA                  3   1912   6282   8648
GdiII       Y!GGCCG                  7   1174   1274   4829   6086   6651
                                         6678   8814
GdiII       C!GGCCR                  6   1139   2241   3239   4829   6086
                                         7372
HaeI        WGG!CCW                 14    738    847    890    895   1197
                                         1534   2168   3146   4406   5969
                                         6262   7548   7559   8011
HgaI        GACGCNNNNN!             12   1009   1324   2181   3172   3354
                                         3951   4098   4587   6892   6971
                                         7644   8222
HgaI        !NNNNNNNNNNGCGTC         5   1086   2610   3907   3944   8952
HgiAI       GWGCW!C                 10   1304   1726   3710   4119   4234
                                         6293   6483   7851   9012   9097
HgiEII      !ACCNNNNNNGGT            3   1952   4998   8113
Hin1I       GR!CGYC                  9    119   1415   2390   3918   4090
                                         6180   6884   6963   8963
HincII      GTY!RAC                  7    123    157    357   2196   2820
                                         4648   7161
HindIII     A!AGCTT                  2    388   1710
HpaI        GTT!AAC                  2   2196   2820
HphI        GGTGANNNNNNNN!           9   1352   2325   2557   2596   3538
                                         3766   5443   8912   9153
HphI        !NNNNNNNTCACC           10   1338   3097   4101   4834   4894
                                         4900   6358   8269   8496   9118
KasI        G!GCGCC                  2   1414   6179
KpnI        GGTAC!C                  2   1720   1954
Ksp632I     CTCTTCN!                 2   7417   9221
Ksp632I     !NNNNGAAGAG              4   1871   4865   6524   6734
KspI        CCGC!GG                  2    730   1077
MamI        GATNN!NNATC              2   3101   6039
MboII       GAAGANNNNNNNN!          10    305   1888   2362   3292   4705
                                         4882   5137   6541   6751   8195
MboII       !NNNNNNNTCTTC           11   1625   1981   4539   5304   6831
                                         7000   7404   8266   9021   9099
                                         9208
MfeI        !CAATTG                  2    283    546
MluI        A!CGCGT                  4    351   3072   3852   4277
MroI        T!CCGGA                  0
MscI        TGG!CCA                  1   6262
MstI        TGC!GCA                  3   1912   6282   8648
MstII       CC!TNAGG                 1   1994
MunI        C!AATTG                  2    284    547
NaeI        GCC!GGC                  4   1178   5340   6682   6967
NarI        GG!CGCC                  2   1415   6180
NcoI        C!CATGG                  2   5877   6612
NdeI        CA!TATG                  1   4727
NgoMI       G!CCGGC                  4   1176   5338   6680   6965
NheI        G!CTAGC                  0
NotI        GC!GGCCGC                1   4829
NruI        TCG!CGA                  2    331   6864
NsiI        ATGCA!T                  3   4889   5720   5792
Nsp(7524)I  RCATG!Y                  7   1113   4533   5143   5718   5790
                                         6585   7537
Nsp(7524)V  TT!CGAA                  1   4850
NspIII      C!YCGRG                  9    521    623    809   1324   1441
                                         3108   4551   4835   5991
NunII       GG!CGCC                  2   1415   6180
PacI        TTAAT!TAA                0
PaeR71      C!TCGAG                  2   1441   4835
PflMI       CCANNNN!NTGG             2   3015   3381
PleI        GAGTCNNNN!               3   1351   5499   7912
PleI        !NNNNNGACTC              8    152   1391   1671   4741   5507
                                         6845   7427   8415
PmaCI       CAC!GTG                  0
PmeI        GTTT!AAAC                1   4921
PmlI        CAC!GTG                  0
PpuMI       RG!GWCCY                 0
PshAI       GACNN!NNGTC              0
PstI        CTGCA!G                  4    792   1297   1760   6233
PvuI        CGAT!CG                  5   1893   2619   3099   3552   8796
PvuII       CAG!CTG                  6   1862   4419   4782   5189   6286
                                         7357
RsrI        G!AATTC                  2   1751   4774
RsrII       CG!GWCCG                 1   6696
SacI        GAGCT!C                  3   1304   1726   3710
SacII       CCGC!GG                  2    730   1077
SalI        G!TCGAC                  3    121    155   7159
SauI        CC!TNAGG                 1   1994
ScaI        AGT!ACT                  1   8906
SexI        !CTCGAG                  2   1440   4834
SfaNI       !NNNNNNNNNGATGC          9    170   2021   2034   4242   5139
                                         6138   6393   6609   8873
SfcI        C!TRYAG                 11    788   1293   1683   1756   2158
                                         4641   5220   6229   7798   7989
                                         8667
SfiI        GGCCNNNN!NGGCC           0
SfuI        TT!CGAA                  1   4850
SgrAI       CR!CCGGYG                0
SinI        G!GWCC                   5    442   3311   6696   8564   8786
SmaI        CCC!GGG                  1   5993
SnaBI       TAC!GTA                  0
SnaI        !GTATAC                  2   4532   7151
SnoI        G!TGCAC                  4   4115   4230   7847   9093
SpeI        A!CTAGT                  1   1734
SphI        GCATG!C                  4   5143   5718   5790   6585
SplI        C!GTACG                  1   4541
SrfI        GCCC!GGGC                0
Sse8387I    CCTGCA!GG                0
SspI        AAT!ATT                  2      4   3000
SstI        GAGCT!C                  3   1304   1726   3710
SstII       CCGC!GG                  2    730   1077
StuI        AGG!CCT                  3    847    890   5969
StyI        C!CWWGG                  4   1560   5877   5970   6612
StySJI      !GAGNNNNNNGTRC           1    473
StySJI      !GYACNNNNNNCTC           1    165
SwaI        ATTT!AAAT                0
Tth111I     GACN!NNGTC               1   6298
Tth111II    CAARCANNNNNNNNNNN!       2   8123   8162
Tth111II    !NNNNNNNNNTGYTTG         5    202    867   4961   6615   8130
Van91I      CCANNNN!NTGG             2   3015   3381
VspI        AT!TAAT                  4   5640   7304   7363   8598
XbaI        T!CTAGA                  0
XcmI        CCANNNNN!NNNNTGG         2   3987   4732
XcyI        C!CCGGG                  1   5991
XhoI        C!TCGAG                  2   1441   4835
XmaI        C!CCGGG                  1   5991
XmaIII      C!GGCCG                  2   4829   6086
XmnI        GAANN!NNTTC              3    775   5641   9025
XorII       CGAT!CG                  5   1893   2619   3099   3552   8796