产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VJC0485 | pGBKT7-53 | Clontech |
酵母双杂交系统
|
询价 | 现货 |
质粒类型:酵母双杂交系统
克隆方法:多克隆位点,限制性内切酶
载体大小:8.3kb
载体标签:c-Myc
载体抗性:Kanamycin (卡那霉素)
载体特点:与pGADT7-T构成Matchmaker Two-Hybrid System 3的双杂交正对照。
pGBKT7-53 encodes a fusion of the murine p53 protein (a.a. 72–390) and the GAL4 DNA-BD (a.a. 1–147). The murine p53 cDNA (GenBank Accession #K01700) was cloned into pGBKT7 at the EcoR I and BamH I sites. The p53 insert was derived from the plasmid described in Iwabuchi et al. (1993); plasmid modification was performed at Clontech. pGBKT7-53 has not been sequenced.