产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VJI0273 | pOG44 | Invitrogen |
基因载体
|
询价 | 现货 |
质粒类型:哺乳细胞表达载体
启动子:CMV
克隆方法:多克隆位点,限制性内切酶
载体大小:5785bp
载体抗性:Ampicillin (氨苄青霉素)
pOG44 is a 5.8 kb Flp recombinase expression vector designed for use with the Flp-In. System (Catalog nos. K6010-01 and K6010-02) available from Invitrogen. When cotransfected with the pcDNA5/FRT plasmid into a Flp-In. mammalian host cell line, the Flp recombinase expressed from pOG44 mediates integration of the pcDNA5/FRT vector containing the gene of interest into the genome via Flp Recombination Target (FRT) sites.
The vector contains the following elements:
·The human cytomegalovirus (CMV) immediate-early enhancer/promoter for highlevel constitutive expression of the Flp recombinase in a wide range of mammalian cells (Andersson et al., 1989; Boshart et al., 1985; Nelson et al., 1987)
·Synthetic intron to enhance expression of the FLP gene (Huang and Gorman, 1990; O’Gorman et al., 1991)
·FLP gene encoding the Flp recombinase (Buchholz et al., 1996) to mediate integration of the pcDNA5/FRT expression plasmid into the genome