质粒类型：昆虫细胞表达载体

高拷贝/低拷贝：高拷贝

启动子：OpIE2

克隆方法：多克隆位点，限制性内切酶

载体大小：3600bp

5' 测序引物及序列：OpIE2-F:5'd[CGCAACGATCTGGTAAACAC]3'

载体标签：HBM (Nterm), V5 (Cterm), His (Cterm)

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Blasticidin

备注：在昆虫细胞中能够稳定表达，HBM标签可实现目的蛋白的分泌表达

pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:

• The OpIE2 promoter for constitutive expression

• The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein

• The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks

• C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin

pMIB/V5-His A&B&C(点击下载)

pMIB/V5-His A&B&C质粒图谱

pMIB v5-His B质粒序列.txt