产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VMA0061 | pSV2-dhfr | ATCC |
Mammalian cell/E.coli shuttle
|
询价 | 现货 |
质粒类型:哺乳动物表达载体
启动子:SV40
表达水平:高
克隆方法:多克隆位点,限制性内切酶
载体大小:4900 bp
5' 测序引物及序列:SV40pro-F:TATTTATGCAGAGGCCGAGG
3' 测序引物及序列:SV40pA-R:GAAATTTGTGATGCTATTGC
载体标签:C-DHFR ( mouse DHFR)
载体抗性:Ampicillin (氨苄青霉素)
筛选标记:MTX (甲氨蝶呤)
备注:pSV2-DHFR载体含有的是小鼠的DHFR基因,同时该载体是哺乳动物细胞和大肠杆菌穿梭质粒,在DHFR细胞系中,可以通过甲氨蝶呤对转染的细胞进行筛选。
A mammalian cell/E.coli shuttle vector. Confers selective advantage to mammalian cell lines grown in the presence of methotrexate. Selectable marker in dhfr- cell culture strains. (ATCC staff) Restriction digests analyzed on agarose gels give the following sizes (kb): BamHI--5.2; PvuII/HindIII--4.8, 0.3; BglII/EcoRI--3.6, 1.5; AccI/HindIII--3.6, 0.54, 0.44, 0.27; AccI--3.6, 0.96, 0.27; HindIII--5.0; PstI--4.1, 0.9. (ATCC staff) Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; HindIII--4.8; BamHI--4.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, E.coli MC1061, vertebrate cells, E.coli.