质粒类型：慢病毒载体

高拷贝/低拷贝：高拷贝

启动子：hUbc

克隆方法：多克隆位点，限制性内切酶

载体大小：9941 bp 

5' 测序引物及序列：hUBCpro-F:TGAAGCTCCGGTTTTGAACT

3' 测序引物及序列：EGFP-N:CGTCGCCGTCCAGCTCGACCAG

载体标签：C-EGFP

载体抗性：Ampicillin (氨苄青霉素)

备注：载体使用Stbl3感受态细胞扩增质粒，可以减少载体基因重组几率

Plasmid pFUGW was constructed by inserting the following into the multicloning site of HR'CS-G: HIV-1 flap sequence PCR-amplified from the HIV NLA4.3 genome, the human polyubiquitin promoter-C (gift of L. Thiel, Amgen), the EGFP gene, and the WRE (woodchuck hepatitis virus posttranscriptional regulatory element) (gift of D. Trono, University of Geneva). Lentiviruses can be produced by cotransfecting the HIV-1 packaging vector Delta8.9 and the VSVG envelope glycoprotein into 293 fibroblasts.

Order of elements: CMV LTR PstI flap PacI Ubiquitin promoter SpeI HindIII PstI SalI XbaI BamHI SmaI KpnI GFP NotI EagI XbaI EcoRI EcoRV HindIII ClaI WRE ClaI SalI XhoI KpnI 3'LTR ApaI PmeI.

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FUGW质粒序列.txt