| 产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
|---|---|---|---|---|---|
| HG-VMA0373 | pSicoR pGK puro | Addgene |
慢病毒载体
|
询价 | 现货 |
质粒类型：慢病毒载体
高拷贝/低拷贝：高拷贝
启动子：CMV
克隆方法：多克隆位点，限制性内切酶
载体大小：7696 bp
5' 测序引物及序列：mU6-F:CAGCACAAAAGGAAACTCACC
载体抗性：Ampicillin (氨苄青霉素)
筛选标记：Puromycin（嘌呤霉素）
备注：本载体利用表达的嘌呤霉素作为筛选标记。嘌呤霉素和shRNA寡核苷酸被LoxP位点加在中间。Cre酶能够同时使得这两个基因在重组过程中切除出载体，同时启动shRNA的表达。
pSicoR PGK Puro allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression. See pSicoR and pSico PGK Puro for more information.
The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map for pSico and on the Jacks Lab website http://web.mit.edu/ccr/labs/jacks/protocols/pSi...
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