质粒类型：哺乳动物表达载体

启动子：CMV IE

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：5.3 kb

5' 测序引物及序列：CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3'

3' 测序引物序列：pIRESrv: 5'-GCCCTAGATGCATGCTCG-3'

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Neomycin (新霉素)

pIRESneo (originally described as pCIN4 by Rees et al.; 1) contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV), which permits the translation of two open reading frames from one messenger RNA (2, 3). After selection with G418, nearly all surviving colonies will stably express the gene of interest, thus decreasing the need to screen large numbers of colonies to find functional clones. The expression cassette of pIRESneo contains the human cytomegalovirus (CMV) major immediate early promoter/enhancer followed by a multiple cloning site (MCS), a synthetic intron known to enhance the stability of the mRNA (4), the ECMV IRES followed by the neomycin phosphotransferase (NPT II) gene, and the polyadenylation signal of the bovine growth hormone. Ribosomes can enter the bicistronic mRNA either at the 5' end to translate the gene of interest or at the ECMV IRES to translate the antibiotic resistance marker.

pIRES-neo(点击下载)