产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VPI0016 | pTracer-CMV2 | Invitrogen |
哺乳动物表达载体
|
询价 | 现货 |
质粒类型:哺乳动物表达载体
启动子:CMV
载体大小:6210 bp
载体抗性:Ampicillin (氨苄青霉素)
筛选标记:Zeocin (博莱霉素)
pTracer-CMV2是一个双表达框载体,CMV启动子后有MCS供插入外源基因;hEF1α启动子调控Cycle 3 GFP-Zeocin fusion的表达,可以做到既有荧光蛋白来做转染指示,且荧光基因表达和外源基因表达又不会互相干扰,而且Zeocin也可以用来做稳定性细胞筛选。另外,由于EM-7启动子的存在,Cycle 3 GFP-Zeocin fusion在E.coli中也可以表达,可以用来做原核荧光定位。
Three pTracer mammalian expression vectors are available that express GFP fused to the selectable marker Zeocin. Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Zeocin fusion.
All three pTracer vectors have the following features:
(1)Cycle 3 GFP-Zeocin fusion for selection in mammalian cell lines
(2)BGH polyA signal and transcription termination sequences to enhance mRNA stability
The pTracer-CMV2 vector offers:
(1)CMV promoter for high-level constitutive expression of your gene of interest
(2)EF-1α promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin fusion
(3)Ampicillin resistance gene for selection in E. coli