质粒类型：哺乳动物表达载体

启动子：CMV

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：10186 bp

5' 测序引物：CMV-F:5'-CGCAAATGGGCGGTAGGCGTG-3'

3' 测序引物：EBV-R:5'-GTGGTTTGTCCAAACTCATC-3'

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Hygromycin (潮霉素)

pCEP4 is an episomal mammalian expression vector that uses the cytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells.

pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.

pCEP4产品说明书.pdf

pCEP4产品手册（Invitrogen）.pdf

pCEP4质粒序列.txt