| 产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
|---|---|---|---|---|---|
| HG-VPI0024 | pUB6/V5 His C | Invitrogen |
哺乳动物表达载体
|
询价 | 现货 |
质粒类型：哺乳动物表达载体
启动子：UbC
克隆方法：多克隆位点，限制性内切酶
载体大小：5459 bp
5' 测序引物及序列：T7 Fwd:5'd[TAATACGACTCACTATAGGG]3'
载体标签：His Tag (6x), V5 Epitope Tag
载体抗性：Ampicillin (氨苄青霉素)
筛选标记：Blasticidin
pUB6/V5-His A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.
The vectors contain the following elements:
Human ubiquitin C promoter (hUbC) for high-level expression across a broad range of species and cell types (Schorpp et al., 1996; Wulff et al., 1990).
Three reading frames to facilitate in-frame cloning with a C-terminal peptide encoding the V5 epitope and a polyhistidine (6×His) metal-binding tag.
Blasticidin resistance gene (bsd) for selection of stable cell lines.
Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7).
The control plasmid, pUB6/V5-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
